Ogasawara H, Kudo T, Kudo M, Ishihara H, Matsuki A
Department of Anesthesiology, University of Hirosaki School of Medicine.
Masui. 1992 Nov;41(11):1723-9.
To elucidate the mechanism of general anesthesia, effects of sevoflurane anesthesia on dopamine metabolism in rat brain were studied. Sevoflurane 3% was administered for 20 minutes to Wistar male rats weighing 230-270 g under spontaneous respiration. The rats were sacrificed by decapitation and the brains were rapidly removed. They were dissected into nine discrete regions, locus coeruleus, pons plus, medulla oblongata, hypothalamus, thalamus, basal ganglia, midbrain, hippocampus, amygdala and cerebral cortex. The contents of dopamine (DA) and its major metabolites, 3, 4-dihydroxyphenyl acetic acid (DOPAC) and homovanillic acid (HVA) were measured by high performance liquid chromatography with the dual-cell coulometric detector before anesthesia, 20 minutes after the start of anesthesia and at the recovery from anesthesia. Significant increases in DA levels were observed in the pons, hypothalamus, thalamus and amygdala by sevoflurane anesthesia as compared with the control group. DOPAC levels increased significantly in the pons, hypothalamus, basal ganglia and cerebral cortex at the recovery from anesthesia. A significant increase in HVA levels was observed in amygdala by sevoflurane anesthesia, while an appreciable decrease in HVA levels was observed in hippocampus at recovery from anesthesia. It is concluded that DA metabolism is significantly suppressed in the pons, hypothalamus, thalamus, basal ganglia, midbrain and amygdala during sevoflurane anesthesia and this change in DA metabolism may be associated with the mechanism of sevoflurane anesthesia.
为阐明全身麻醉的机制，研究了七氟醚麻醉对大鼠脑内多巴胺代谢的影响。对体重230 - 270 g的雄性Wistar大鼠在自主呼吸下给予3%七氟醚20分钟。通过断头处死大鼠并迅速取出大脑。将其解剖为九个离散区域，即蓝斑、脑桥加、延髓、下丘脑、丘脑、基底神经节、中脑、海马、杏仁核和大脑皮层。在麻醉前、麻醉开始后20分钟以及麻醉恢复时，使用配备双池库仑检测器的高效液相色谱法测量多巴胺（DA）及其主要代谢产物3,4 - 二羟基苯乙酸（DOPAC）和高香草酸（HVA）的含量。与对照组相比，七氟醚麻醉后，脑桥、下丘脑、丘脑和杏仁核中的DA水平显著升高。麻醉恢复时，脑桥、下丘脑、基底神经节和大脑皮层中的DOPAC水平显著升高。七氟醚麻醉后杏仁核中的HVA水平显著升高，而麻醉恢复时海马中的HVA水平明显降低。结论是，七氟醚麻醉期间，脑桥、下丘脑、丘脑、基底神经节、中脑和杏仁核中的DA代谢受到显著抑制，这种DA代谢的变化可能与七氟醚麻醉的机制有关。
