Ogasawara H, Takahashi S, Kudo T, Kudo M, Ishihara H, Matsuki A
Department of Anesthesiology, University of Hirosaki School of Medicine.
Masui. 1993 Oct;42(10):1412-7.
To elucidate the mechanism of general anesthesia, effects of sevoflurane anesthesia on serotonin metabolism in rat brain were studied. Three percent sevoflurane was administered for twenty minutes to Wistar male rats weighing 230-270g under spontaneous respiration. The rats were sacrificed by decapitation and the brains were rapidly removed. They were dissected into nine discrete regions, locus coeruleus, pons plus medulla oblongata, hypothalamus, thalamus, basal ganglia, midbrain, hippocampus, amygdala and cerebral cortex. The contents of serotonin (5-HT) and one of its major metabolites, 5-hydroxyindole-3-acetic acid (5-HIAA) were measured by high performance liquid chromatography with the dual-cell coulometric detector before anesthesia, 20 minutes after the start of anesthesia and at the recovery from anesthesia. Significant increases in 5-HT levels were observed in the pons, hypothalamus, midbrain, amygdala and cerebral cortex by sevoflurane anesthesia as compared with the control group. 5-HIAA levels decreased significantly in the thalamus by sevoflurane anesthesia, while an appreciable increase in 5-HIAA levels was observed in the basal ganglia at the recovery from anesthesia. It is concluded that 5-HT metabolism is significantly suppressed in the pons, hypothalamus, thalamus, midbrain, amygdala and cerebral cortex during sevoflurane anesthesia and this change in 5-HT metabolism may be associated with a mechanism of sevoflurane anesthesia.
为阐明全身麻醉的机制，研究了七氟醚麻醉对大鼠脑内5-羟色胺代谢的影响。对体重230 - 270g的雄性Wistar大鼠在自主呼吸状态下给予3%七氟醚20分钟。大鼠断头处死，迅速取出大脑。将大脑解剖为九个离散区域，即蓝斑、脑桥加延髓、下丘脑、丘脑、基底神经节、中脑、海马、杏仁核和大脑皮层。在麻醉前、麻醉开始后20分钟及麻醉苏醒时，采用配备双池库仑检测器的高效液相色谱法测定5-羟色胺（5-HT）及其主要代谢产物之一5-羟吲哚-3-乙酸（5-HIAA）的含量。与对照组相比，七氟醚麻醉后在脑桥、下丘脑、中脑、杏仁核和大脑皮层观察到5-HT水平显著升高。七氟醚麻醉使丘脑的5-HIAA水平显著降低，而在麻醉苏醒时基底神经节的5-HIAA水平明显升高。得出结论，在七氟醚麻醉期间，脑桥、下丘脑、丘脑、中脑、杏仁核和大脑皮层的5-HT代谢受到显著抑制，5-HT代谢的这种变化可能与七氟醚麻醉的机制有关。
