Shah Miti M, Fujiyama Kazuhito, Flynn C Robert, Joshi Lokesh
Harrington Department of Bioengineering and Arizona BioDesign Institute, Arizona State University, PO Box 879709, Tempe, Arizona 85287-9709, USA.
Nat Biotechnol. 2003 Dec;21(12):1470-1. doi: 10.1038/nbt912. Epub 2003 Nov 9.
Bioengineered plants are emerging as promising systems for the production of therapeutically valuable proteins. It has been commonly accepted that plants do not perform mammalian-like post-translational modifications, particularly sialylation of glycoconjugates, and no evidence has previously been reported to suggest that they have such capabilities. Here we report the presence of sialylated glycoconjugates in suspension-cultured cells of Arabidopsis thaliana and suggest that a genetic and enzymatic basis for sialylation exists in plants.
生物工程植物正成为生产具有治疗价值蛋白质的有前景的系统。人们普遍认为植物不会进行类似哺乳动物的翻译后修饰，尤其是糖缀合物的唾液酸化，而且此前没有证据表明它们具有这种能力。在此，我们报告了拟南芥悬浮培养细胞中存在唾液酸化的糖缀合物，并表明植物中存在唾液酸化的遗传和酶学基础。
