Fraunhofer USA Center for Molecular Biotechnology, Newark, DE, USA.
FEBS Open Bio. 2011 Oct 30;1:15-22. doi: 10.1016/j.fob.2011.10.003. Print 2011 Dec.
Green algae have a great potential as biofactories for the production of proteins. Chlamydomonas reinhardtii, a representative of eukaryotic microalgae, has been extensively used as a model organism to study light-induced gene expression, chloroplast biogenesis, photosynthesis, light perception, cell-cell recognition, and cell cycle control. However, little is known about the glycosylation machinery and N-linked glycan structures of green algae. In this study, we performed mass spectrometry analysis of N-linked oligosaccharides released from total extracts of Chlamydomonas reinhardtii and demonstrated that C. reinhardtii algae possess glycoproteins with mammalian-like sialylated N-linked oligosaccharides. These findings suggest that C. reinhardtii may be an attractive system for expression of target proteins.
绿藻具有作为生物工厂生产蛋白质的巨大潜力。莱茵衣藻是真核微藻的代表，被广泛用作研究光诱导基因表达、叶绿体发生、光合作用、光感知、细胞间识别和细胞周期控制的模式生物。然而，关于绿藻的糖基化机制和 N-连接聚糖结构知之甚少。在这项研究中，我们对来自莱茵衣藻总提取物的 N-连接寡糖进行了质谱分析，证明莱茵衣藻藻类含有具有类似哺乳动物唾液酸化的 N-连接寡糖的糖蛋白。这些发现表明，莱茵衣藻可能是表达靶蛋白的有吸引力的系统。
