O'Toole Eileen T, McDonald Kent L, Mäntler Jana, McIntosh J Richard, Hyman Anthony A, Müller-Reichert Thomas
Boulder Laboratory for 3-D Electron Microscopy of Cells, University of Colorado, 80309, USA.
J Cell Biol. 2003 Nov 10;163(3):451-6. doi: 10.1083/jcb.200304035.
During mitosis, the connections of microtubules (MTs) to centrosomes and kinetochores are dynamic. From in vitro studies, it is known that the dynamic behavior of MTs is related to the structure of their ends, but we know little about the structure of MT ends in spindles. Here, we use high-voltage electron tomography to study the centrosome- and kinetochore-associated ends of spindle MTs in embryonic cells of the nematode, Caenorhabditis elegans. Centrosome-associated MT ends are either closed or open. Closed MT ends are more numerous and are uniformly distributed around the centrosome, but open ends are found preferentially on kinetochore-attached MTs. These results have structural implications for models of MT interactions with centrosomes.
在有丝分裂期间，微管（MTs）与中心体和动粒的连接是动态的。从体外研究可知，MTs的动态行为与其末端结构有关，但我们对纺锤体中MT末端的结构了解甚少。在这里，我们使用高压电子断层扫描技术来研究线虫秀丽隐杆线虫胚胎细胞中纺锤体MTs与中心体和动粒相关的末端。与中心体相关的MT末端要么是封闭的，要么是开放的。封闭的MT末端数量更多，且均匀分布在中心体周围，但开放末端优先出现在与动粒相连的MTs上。这些结果对MT与中心体相互作用的模型具有结构上的启示意义。
