Department of Medicine, University of Pennsylvania, University of Pennsylvania School of Medicine, 415 Curie Blvd., Philadelphia, PA 19104, USA.
Curr Opin Chem Biol. 2010 Feb;14(1):37-42. doi: 10.1016/j.cbpa.2009.10.012. Epub 2009 Nov 10.
Cellular processes are orchestrated by the precise coordination and regulation of molecular events in the cell. Fluorescent protein-based biosensors coupled with live-cell imaging have enabled the visualization of these events in real time and helped shape some of the current concepts of signal transduction, such as spatial compartmentation. The quantitative information produced by these tools has been incorporated into mathematical models that are capable of predicting highly complex and dynamic behaviors of cellular signaling networks, thus providing a systems level understanding of how pathways interact to produce a functional response. Finally, with technological advances in high-throughput and in vivo imaging, these molecular tools promise to continually engender significant contributions to our understanding of cellular processes under normal and diseased conditions.
细胞过程是通过细胞内分子事件的精确协调和调节来实现的。基于荧光蛋白的生物传感器与活细胞成像相结合，使这些事件能够实时可视化，并帮助形成了一些当前的信号转导概念，如空间分隔。这些工具产生的定量信息已被纳入数学模型中，这些模型能够预测细胞信号网络的高度复杂和动态行为，从而提供了对途径如何相互作用以产生功能响应的系统水平理解。最后，随着高通量和体内成像技术的进步，这些分子工具有望不断为我们在正常和疾病条件下对细胞过程的理解做出重要贡献。
