Januszkiewicz Danuta, Wysoki Jacek, Lewandowski Krzysztof, Pernak Monika, Nowicka Karina, Rembowska Jolanta, Nowak Jerzy
Institute of Human Genetics PAN, Polish Academy of Sciences, Strzeszynska 32, 60-479 Poznan, Poland.
Int J Mol Med. 2003 Dec;12(6):935-8.
The expression of three components of telomerase complex (hTR, hTERT, TP1) along with telomerase activity and telomere length in leukemic cells was investigated. Cells were isolated from peripheral blood and/or bone marrow of children with acute lymphoblastic (ALL) and non-lymphoblastic (ANLL) leukemia. Expression of three components of telomerase as well as telomerase activity was found in all leukemic cells. Chemiluminescent detection of terminal restriction fragments (TRF) from DNA isolated from ALL cells showed variable patterns expressing considerable heterogeneity of telomere length. The ALL cells appeared to have both long and short telomere lengths, in contrast to normal peripheral lymphocytes, which produced limited pattern of TRF. The ANLL cells produced predominantly short telomere pattern despite high telomerase activity and expression. It can be concluded that high telomerase activity and expression in leukemic cells is not always correlated with long telomeres (TRF pattern).
研究了白血病细胞中端粒酶复合物的三个组分（hTR、hTERT、TP1）的表达以及端粒酶活性和端粒长度。细胞取自急性淋巴细胞白血病（ALL）和非淋巴细胞白血病（ANLL）患儿的外周血和/或骨髓。在所有白血病细胞中均发现了端粒酶三个组分的表达以及端粒酶活性。对从ALL细胞中分离的DNA进行末端限制片段（TRF）的化学发光检测，结果显示出不同的模式，表明端粒长度存在显著异质性。与正常外周淋巴细胞产生有限的TRF模式不同，ALL细胞似乎同时具有长端粒和短端粒长度。尽管ANLL细胞具有较高的端粒酶活性和表达，但主要产生短端粒模式。可以得出结论，白血病细胞中的高端粒酶活性和表达并不总是与长端粒（TRF模式）相关。
