甘草甜素可增强肝炎小鼠肝脏树突状细胞的白细胞介素-10生成。
Glycyrrhizin enhances interleukin-10 production by liver dendritic cells in mice with hepatitis.
作者信息
Abe Masanori, Akbar Fazle, Hasebe Aki, Horiike Norio, Onji Morikazu
机构信息
Third Department of Internal Medicine, Ehime University School of Medicine, 791-0295, Ehime, Shigenobu-cho, Japan.
出版信息
J Gastroenterol. 2003;38(10):962-7. doi: 10.1007/s00535-003-1179-7.
BACKGROUND
Glycyrrhizin (GL), an aqueous extract of licorice root, is known to have various immune-modulating and biological response-modifier activities. GL is used in patients with hepatitis to reduce the activity of liver inflammation; however, the mechanism underlying the anti-inflammatory activity of GL is poorly understood. As antigen-presenting dendritic cells (DC) in the tissue play a major role in the regulation of the inflammatory mucosal milieu during tissue inflammation, we studied whether the function of liver DC was altered by GL therapy in a murine model of concanavalin-A (con A)-induced hepatitis.
METHODS
Liver DC were propagated from control mice or mice with Con-A-induced hepatitis, and the effect of GL on liver DC was evaluated in vivo and in vitro.
RESULTS
The levels of interleukin (IL)-10 produced by liver DC were significantly lower in mice with Con-A-induced hepatitis compared with control mice. However, treatment with GL caused increased production of IL-10 in mice with Con A-induced hepatitis. The increased production of IL-10 by mice with Con A-induced hepatitis was also confirmed in vitro by culturing liver DC with GL.
CONCLUSIONS
This study indicates that increased production of IL-10 by liver DC due to GL administration may be involved in downregulation of the levels of liver inflammation in mice with Con A-induced hepatitis. Glycyrrhizin (GL), an aqueous extract of licorice root, is known to have various immune-modulating and biological response-modifier activities. GL is used in patients with hepatitis to reduce the activity of liver inflammation; however, the mechanism underlying the anti-inflammatory activity of GL is poorly understood. As antigen-presenting dendritic cells (DC) in the tissue play a major role in the regulation of the inflammatory mucosal milieu during tissue inflammation, we studied whether the function of liver DC was altered by GL therapy in a murine model of concanavalin-A (Con A)-induced hepatitis.
背景
甘草甜素（GL）是甘草根的水提取物，已知具有多种免疫调节和生物反应调节活性。GL用于治疗肝炎患者以减轻肝脏炎症活动；然而，GL抗炎活性的潜在机制尚不清楚。由于组织中的抗原呈递树突状细胞（DC）在组织炎症期间调节炎症性黏膜环境中起主要作用，我们在伴刀豆球蛋白A（Con A）诱导的肝炎小鼠模型中研究了GL治疗是否会改变肝脏DC的功能。
方法
从对照小鼠或Con A诱导的肝炎小鼠中培养肝脏DC，并在体内和体外评估GL对肝脏DC的影响。
结果
与对照小鼠相比，Con A诱导的肝炎小鼠肝脏DC产生的白细胞介素（IL）-10水平显著降低。然而，用GL治疗可使Con A诱导的肝炎小鼠的IL-10产生增加。通过用GL培养肝脏DC，在体外也证实了Con A诱导的肝炎小鼠IL-10产生增加。
结论
本研究表明，给予GL后肝脏DC产生的IL-10增加可能参与了Con A诱导的肝炎小鼠肝脏炎症水平的下调。甘草甜素（GL）是甘草根的水提取物，已知具有多种免疫调节和生物反应调节活性。GL用于治疗肝炎患者以减轻肝脏炎症活动；然而，GL抗炎活性的潜在机制尚不清楚。由于组织中的抗原呈递树突状细胞（DC）在组织炎症期间调节炎症性黏膜环境中起主要作用，我们在伴刀豆球蛋白A（Con A）诱导的肝炎小鼠模型中研究了GL治疗是否会改变肝脏DC的功能。
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