Department of Immunology, School of Basic Medical Science, China Medical University, No.92, North Second Road, Heping District, Shenyang 110001, PR China.
Int Immunopharmacol. 2012 Mar;12(3):518-25. doi: 10.1016/j.intimp.2012.01.006. Epub 2012 Jan 29.
The aim of this study is to investigate phenotypic and functional modulation of murine dendritic cells (DCs) with use of purified Glycyrrhizin (GL). These impacts of GL on DCs both from bone marrow derived DCs and established DC cell 2.4 were assessed with conventional scanning electron microscopy (SEM), flow cytometry (FCM), transmission electron microscopy (TEM), cytochemistry assay, FITC-dextran, bio-assay and enzyme linked immunosorbent assay (ELISA). We found that the purified GL induced phenotypic maturation as evidenced by increased expression of CD86, CD40, CD80, CD83 and major histocompatibility complex II (MHC II). The functional tests showed the activity of acidic phosphatase (ACP) inside the DCs2.4 cells were down- regulated after treatment with GL (which occurs when phagocytosis of DCs2.4 cells were decreased). Finally, we proved that GL increased the production of IL-12, IL-10 and decreased the production of tumor necrosis factor alpha (TNF-α). These data indicated that GL could promote maturation of DCs and this adjuvant-like activity may have potential therapeutic value. It is therefore concluded that GL could exert positive modulation on murine DCs.
本研究旨在探讨纯化甘草酸（GL）对鼠树突状细胞（DC）的表型和功能调节作用。通过常规扫描电子显微镜（SEM）、流式细胞术（FCM）、透射电子显微镜（TEM）、细胞化学测定、FITC-葡聚糖、生物测定和酶联免疫吸附试验（ELISA）评估 GL 对骨髓来源的 DC 和已建立的 DC 细胞 2.4 的影响。我们发现，纯化的 GL 诱导表型成熟，表现为 CD86、CD40、CD80、CD83 和主要组织相容性复合物 II（MHC II）表达增加。功能测试显示，GL 处理后 DCs2.4 细胞内酸性磷酸酶（ACP）的活性下调（这发生在 DCs2.4 细胞吞噬作用减少时）。最后，我们证明 GL 增加了白细胞介素 12（IL-12）的产生，减少了肿瘤坏死因子 alpha（TNF-α）的产生。这些数据表明 GL 可以促进 DC 的成熟，这种佐剂样活性可能具有潜在的治疗价值。因此，可以得出结论，GL 可以对鼠树突状细胞发挥正向调节作用。
