Kotajima Nobuo, Ishizaka Miki, Oshitani Setsuko, Fukumura Yukihito, Ushijima Yoshio, Murakami Masami
Department of Clinical Laboratory Medicine and Clinical Laboratory Center, Gunma University School of Medicine, Gunma, Japan.
J Clin Lab Anal. 2003;17(6):223-8. doi: 10.1002/jcla.10096.
Ferritin is an indispensable parameter in the diagnosis of latent iron deficiency anemia or siderosis. In this study, we evaluated the utility of a reagent for ferritin measurement in a latex agglutination (LA) test, using general chemistry analyzers. The intraassay coefficient of variation (CV) was 0.8-3.4% and the interassay CV was 0.0-0.7%. Linearity was observed up to 1100 ng/mL. The effective sensitivity value was 4.0 ng/mL. In addition, good results were obtained with the prozone test, the effects of interferences, correlation with the enzyme immunoassay (EIA) method, and functional assay sensitivity. A significant positive correlation with C-reactive protein (r=0.586, P<0.001) was found. When compared with liver-related biochemical parameters (asparate aminotransferase (AST) and alanine aminotransferase (ALT)) in patients with impaired liver function, ferritin varied in parallel with the liver-related parameters. This assay system was able to measure ferritin accurately over a wide range, and thus could be used to diagnose cancer, siderosis, and iron deficiency anemia. The LA assay system can be employed for measurement with general chemistry analyzers, with rapid and convenient execution. In addition, the LA test allows the simultaneous measurement of other markers of iron deficiency anemia, so clinicians can rapidly obtain examination results.
铁蛋白是诊断潜在缺铁性贫血或铁沉着症不可或缺的参数。在本研究中，我们使用常规化学分析仪评估了一种用于乳胶凝集（LA）试验中铁蛋白测定的试剂的效用。批内变异系数（CV）为0.8 - 3.4%，批间CV为0.0 - 0.7%。在高达1100 ng/mL时观察到线性关系。有效灵敏度值为4.0 ng/mL。此外，在前带试验、干扰影响、与酶免疫测定（EIA）方法的相关性以及功能测定灵敏度方面均获得了良好结果。发现与C反应蛋白存在显著正相关（r = 0.586，P < 0.001）。与肝功能受损患者的肝脏相关生化参数（天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT））相比，铁蛋白与肝脏相关参数呈平行变化。该检测系统能够在较宽范围内准确测量铁蛋白，因此可用于诊断癌症、铁沉着症和缺铁性贫血。LA检测系统可用于常规化学分析仪进行测量，操作快速便捷。此外，LA试验允许同时测量缺铁性贫血的其他标志物，因此临床医生可以快速获得检查结果。
