Bernard A M, Lauwerys R R
Clin Chem. 1983 Jun;29(6):1007-11.
Latex immunoassay is a nonisotopic method based on agglutination, by protein, of calibrated latex particles coated with a specific antibody. The assay has been automated in a simple continuous-flow system by incubating the reaction mixture in a heated mixing coil for 25 min and measuring the agglutination with a cell counter. No external shaking of the latex suspension and no additional reagent is required for the agglutination. The method can accurately and precisely quantify a wide variety of proteins in plasma and urine, including human ferritin, beta 2-microglobulin, retinol-binding protein, and albumin. Depending on the antigen-antibody system, the detection limit ranges from 10(-10) to about 10(-12) mol/L. Within- and between-assay CVs are less than 10%. In the assay of ferritin, sera are pretreated to eliminate interferences from chylomicrons, complement, and rheumatoid factor.
乳胶免疫测定是一种基于凝集反应的非同位素方法，通过蛋白质使包被有特异性抗体的校准乳胶颗粒发生凝集。该测定已在一个简单的连续流动系统中实现自动化，即将反应混合物在加热的混合盘管中孵育25分钟，并用细胞计数器测量凝集情况。凝集过程中无需对乳胶悬浮液进行外部振荡，也不需要额外的试剂。该方法能够准确且精确地定量血浆和尿液中的多种蛋白质，包括人铁蛋白、β2-微球蛋白、视黄醇结合蛋白和白蛋白。根据抗原-抗体系统的不同，检测限范围为10^(-10)至约l0^(-12) mol/L。批内和批间变异系数均小于10%。在铁蛋白测定中，血清需经过预处理以消除乳糜微粒、补体和类风湿因子的干扰。
