Sharp Christopher D, Fowler M, Jackson T H, Houghton J, Warren A, Nanda A, Chandler I, Cappell B, Long A, Minagar A, Alexander J S
Department of Molecular and Cellular Physiology, LSU Health Sciences Center, Shreveport, LA, USA.
BMC Neurosci. 2003 Nov 13;4:28. doi: 10.1186/1471-2202-4-28.
L-glutamate, an excitatory neurotransmitter, binds to both ionotropic and metabotropic glutamate receptors. In certain parts of the brain the BBB contains two normally impermeable barriers: 1) cerebral endothelial barrier and 2) cerebral epithelial barrier. Human cerebral endothelial cells express NMDA receptors; however, to date, human cerebral epithelial cells (neuroepithelial cells) have not been shown to express NMDA receptor message or protein. In this study, human hypothalamic sections were examined for NMDA receptors (NMDAR) expression via immunohistochemistry and murine neuroepithelial cell line (V1) were examined for NMDAR via RT-PCR and Western analysis. We found that human cerebral epithelium express protein and cultured mouse neuroepithelial cells express both mRNA and protein for the NMDA receptor. These findings may have important consequences for neuroepithelial responses during excitotoxicity and in disease.
L-谷氨酸是一种兴奋性神经递质，可与离子型和代谢型谷氨酸受体结合。在大脑的某些部位，血脑屏障包含两个通常不可渗透的屏障：1）脑内皮屏障和2）脑上皮屏障。人脑血管内皮细胞表达NMDA受体；然而，迄今为止，尚未发现人脑上皮细胞（神经上皮细胞）表达NMDA受体信息或蛋白质。在本研究中，通过免疫组织化学检查人下丘脑切片中NMDA受体（NMDAR）的表达，并通过RT-PCR和蛋白质印迹分析检查小鼠神经上皮细胞系（V1）中的NMDAR。我们发现人脑上皮表达蛋白质，培养的小鼠神经上皮细胞表达NMDA受体的mRNA和蛋白质。这些发现可能对兴奋毒性期间和疾病中的神经上皮反应具有重要影响。
