Savarino L, Breschi L, Tedaldi M, Ciapetti G, Tarabusi C, Greco M, Giunti A, Prati C
Laboratory for Pathophysiology of Orthopaedic Implants, Rizzoli Orthopaedic Institute & Department of Anatomical Sciences and Skeletal Muscle Pathophysiology, University of Bologna, Via di Barbiano 1/10, 40136 Bologna, Italy.
Biomaterials. 2004 Mar;25(6):1011-7. doi: 10.1016/s0142-9612(03)00628-8.
The study aimed to define the in vitro secondary caries inhibiting potential of restorative materials currently used in dental practice. Class V restorations were prepared in extracted human third molars and immersed in a demineralizing solution (lactic acid, pH 4.5) at 37 degrees C for 2 days to simulate secondary caries formation. The bonding and the restorative systems tested in the study were: Scotchbond 1+Z 250 (Group A), Scotchbond 1+F 2000 (Group B), ABF+APX (Group C), ABF+F2000 (Group D). Perimarginal dentine, immediately close to the margin of the restoration, and exposed dentine, at approximately 0.5 mm from the margins of the restoration, after exposure to the acid solution, were investigated; protected dentine, at approximately 4 mm from the margin in a varnish-covered area, was analysed as control. Polarized light microscopy and contact transverse microradiography (TMR) were employed. The output parameters were lesion shape and size (depth in microm) of the exposed dentine, dentine mineral volume%, and integrated mineral loss (Delta Z, in %volmicrom) of the lesions. Compomers (Groups B and D) showed a thinner demineralization of the outer lesions, a less demineralization along the perimarginal dentine (inner lesion) and more caries inhibition zones or CIZs (Delta Z positive values) compared to composites (Groups A and C). In conclusion, Groups B and D materials seemed to partially counteract the marginal demineralization induced by an acid solution and favourably influence the formation of CIZs along the restorations. On the contrary, composites did not show a protective effect, probably due to an insufficient marginal seal and the lack of fluoride release.
该研究旨在确定目前牙科临床使用的修复材料的体外继发龋抑制潜力。在拔除的人类第三磨牙上制备V类洞修复体，并将其置于37摄氏度的脱矿溶液（乳酸，pH值4.5）中浸泡2天，以模拟继发龋形成。该研究中测试的粘结和修复系统为：Scotchbond 1+Z 250（A组）、Scotchbond 1+F 2000（B组）、ABF+APX（C组）、ABF+F2000（D组）。在酸溶液处理后，对紧邻修复体边缘的边缘牙本质以及距修复体边缘约0.5毫米处的暴露牙本质进行研究；将距边缘约4毫米的涂有清漆区域内的受保护牙本质作为对照进行分析。采用偏光显微镜和接触横向微放射照相术（TMR）。输出参数为暴露牙本质的病变形状和大小（深度，单位为微米）、牙本质矿物质体积百分比以及病变的累计矿物质损失（ΔZ，单位为%vol·μm）。与复合树脂（A组和C组）相比，聚羧酸锌水门汀（B组和D组）显示外层病变脱矿更薄，边缘牙本质（内层病变）脱矿更少，且龋抑制区或CIZs（ΔZ正值）更多。总之，B组和D组材料似乎部分抵消了酸溶液引起的边缘脱矿，并对沿修复体的CIZs形成产生有利影响。相反，复合树脂未显示出保护作用，可能是由于边缘封闭不足和缺乏氟释放。
