Pods R, Ross D, van Hülst S, Rudack C, Maune St
Department of Otorhinolaryngology, Head- and Neck-Surgery, University Kiel, Kiel, Germany.
Allergy. 2003 Nov;58(11):1165-70. doi: 10.1034/j.1398-9995.2003.00276.x.
Polyposis, asthma, aspirin-intolerance and aspirin-triad are mostly accompanied with eosinophilia of mucosal airways. Chemotactic cytokines, the CC-chemokines regulated on activation, normal T-cell expressed (RANTES), eotaxin, and eotaxin-2 activate and attract eosinophilic leukocytes to the site of inflammation. This points to the implication of CC-chemokines in eosinophilia of nasal tissue of these diseases.
Therefore, nasal polypous tissue specimens of patients suffering from chronic nasal polypous sinusitis (NP), intrinsic asthma (ATA), aspirin-intolerance (AINA), and aspirin-triad (TRIAD) were investigated. The amount of mRNA and protein of CC-chemokines was analyzed using semi-quantitative reverse transcriptase polymerase chain reaction and chemokine-specific enzyme-immuno-assays. The patterns of CC-chemokines were compared.
The mRNA-expression as well as protein synthesis of CC-chemokines was quantified in all tissues investigated. The expression of RANTES-mRNA in NP, ATA, AINA, and TRIAD (averaging 148-324% D-glyceraldehyde-3-phosphate dehydrogenase) and protein synthesis (0.13-0.15 ng/mg tissue weight) did not differ significantly. But the protein synthesis of eotaxin- and eotaxin-2-mRNA was significantly (P < 0.05) higher in TRIAD (3.3 pg/mg and 3.4 ng/mg tissue weight) (4 ng/mg tissue weight), than in NP, ATA, or AINA (1.8 pg/mg and 2.1 ng/mg, 2.1 pg/mg and 1.6 ng/mg, or 1.7 pg/mg and 2.2 ng/mg tissue weight, respectively).
Patients suffering from TRIAD in association with tissue eosinophilia were characterized by elevated eotaxin and eotaxin-2 mRNA-expression as well as protein-synthesis. This pointed to the implication of eotaxins and RANTES in eosinophilia-associated diseases. Further studies will have to prove, whether the analysis of these chemokines might improve the diagnosis of eosinophilia associated polyposis and initiate the development of new therapeutic strategies.
息肉病、哮喘、阿司匹林不耐受和阿司匹林三联征大多伴有气道黏膜嗜酸性粒细胞增多。趋化细胞因子，即活化调节的CC趋化因子、正常T细胞表达的RANTES、嗜酸性粒细胞趋化因子和嗜酸性粒细胞趋化因子-2，可激活并吸引嗜酸性粒细胞至炎症部位。这表明CC趋化因子与这些疾病鼻组织中的嗜酸性粒细胞增多有关。
因此，对患有慢性鼻息肉鼻窦炎（NP）、内源性哮喘（ATA）、阿司匹林不耐受（AINA）和阿司匹林三联征（TRIAD）的患者的鼻息肉组织标本进行了研究。使用半定量逆转录聚合酶链反应和趋化因子特异性酶免疫测定法分析CC趋化因子的mRNA和蛋白质含量。比较CC趋化因子的模式。
对所有研究组织中CC趋化因子的mRNA表达和蛋白质合成进行了定量。NP、ATA、AINA和TRIAD中RANTES-mRNA的表达（平均148 - 324% D-甘油醛-3-磷酸脱氢酶）和蛋白质合成（0.13 - 0.15 ng/mg组织重量）无显著差异。但TRIAD中嗜酸性粒细胞趋化因子和嗜酸性粒细胞趋化因子-2-mRNA的蛋白质合成（分别为3.3 pg/mg和3.4 ng/mg组织重量）（4 ng/mg组织重量）显著高于NP、ATA或AINA（分别为1.8 pg/mg和2.1 ng/mg、2.1 pg/mg和1.6 ng/mg或1.7 pg/mg和2.2 ng/mg组织重量）（P < 0.05）。
患有与组织嗜酸性粒细胞增多相关的TRIAD的患者，其特征为嗜酸性粒细胞趋化因子和嗜酸性粒细胞趋化因子-2 mRNA表达及蛋白质合成升高。这表明嗜酸性粒细胞趋化因子和RANTES与嗜酸性粒细胞增多相关疾病有关。进一步的研究将必须证明，对这些趋化因子的分析是否可能改善嗜酸性粒细胞增多相关息肉病的诊断，并启动新治疗策略的开发。
