Chiariotti L, Berlingieri M T, De Rosa P, Battaglia C, Berger N, Bruni C B, Fusco A
Dipartimento di Biologia e Patologia Cellulare e Molecolare L. Califano, Università degli Studi di Napoli, Italy.
Oncogene. 1992 Dec;7(12):2507-11.
Murine beta-galactoside-binding protein has been shown to be a cell growth regulatory molecule and a cytostatic factor. We analysed the beta-galactoside-binding protein gene expression in a thyroid cell system including two normal cell lines (FRTL-5 and PC Cl 3) and the same cells transfected by several oncogenes that induce different degrees of malignancy and differentiation. We show that beta-galactoside-binding protein mRNA levels correlate with the expression of the malignant phenotype. Run-on experiments suggest that a transcriptional effect accounts at least in part for such a difference. We also show that the beta-galactoside-binding protein gene expression is increased in most human papillary thyroid carcinomas compared with normal thyroid.
鼠β-半乳糖苷结合蛋白已被证明是一种细胞生长调节分子和细胞生长抑制因子。我们分析了β-半乳糖苷结合蛋白基因在一个甲状腺细胞系统中的表达，该系统包括两个正常细胞系（FRTL-5和PC Cl 3）以及由几种诱导不同程度恶性和分化的癌基因转染的相同细胞。我们发现β-半乳糖苷结合蛋白mRNA水平与恶性表型的表达相关。核转录实验表明，转录效应至少部分地解释了这种差异。我们还表明，与正常甲状腺相比，大多数人甲状腺乳头状癌中β-半乳糖苷结合蛋白基因的表达增加。
