Califano D, Monaco C, Santelli G, Giuliano A, Veronese M L, Berlingieri M T, de Franciscis V, Berger N, Trapasso F, Santoro M, Viglietto G, Fusco A
Servizio di Oncologia Sperimentale E, Istituto per lo Studio e la Cura dei Tumori Fondazione G. Pascale, Napoli, Italy.
Cancer Res. 1998 Feb 15;58(4):823-8.
A subtractive thyroid cDNA library was constructed from two human thyroid carcinoma cell lines originating from an anaplastic carcinoma and a papillary thyroid carcinoma. The library was used to identify genes correlated with the progression to a highly malignant phenotype. The thymosin beta-10 gene was isolated and found to be expressed at much higher levels in the anaplastic cell line than in the papillary cells. The thymosin beta-10 gene was overexpressed in five carcinoma cell lines compared with normal thyroid tissue and normal thyroid primary culture cells. The highest expression occurred in the most malignant cell lines. Thymosin beta-10 gene expression was also increased in surgically removed human thyroid carcinomas and was highest in the anaplastic carcinomas. Thymosin beta-10 gene expression was correlated with the degree of the malignant phenotype also in rat thyroid cells transfected with cellular and viral oncogenes of different tumorigenicity. These results show that thymosin beta-10 overexpression is a general event of thyroid cell neoplastic transformation and suggest that the gene is involved in the progression of thyroid carcinogenesis. Finally, the thymosin beta-10 gene was located on chromosome 2q37 by fluorescence in situ hybridization analysis.
从源自未分化癌和甲状腺乳头状癌的两个人类甲状腺癌细胞系构建了一个消减甲状腺cDNA文库。该文库用于鉴定与向高恶性表型进展相关的基因。分离出胸腺素β-10基因，发现其在未分化细胞系中的表达水平远高于乳头状细胞。与正常甲状腺组织和正常甲状腺原代培养细胞相比，胸腺素β-10基因在五种癌细胞系中过表达。在最恶性的细胞系中表达最高。在手术切除的人类甲状腺癌中，胸腺素β-10基因表达也增加，在未分化癌中最高。在用具有不同致瘤性的细胞和病毒癌基因转染的大鼠甲状腺细胞中，胸腺素β-10基因表达也与恶性表型程度相关。这些结果表明胸腺素β-10过表达是甲状腺细胞肿瘤转化的普遍事件，并提示该基因参与甲状腺癌发生的进展。最后，通过荧光原位杂交分析将胸腺素β-10基因定位在2q37染色体上。
