Bourne N, Zaneveld L J D, Ward J A, Ireland J P, Stanberry L R
Division of Infectious Diseases, Children's Hospital Medical Center, Cincinnati, OH, USA.
Clin Microbiol Infect. 2003 Aug;9(8):816-22. doi: 10.1046/j.1469-0691.2003.00659.x.
To investigate the in vivo activity of poly(sodium 4-styrene sulfonate) (T-PSS) gel formulations as topical microbicides.
The ability of the gel formulations to reduce the incidence of infection when applied prior to pathogen challenge was examined in mouse models of vaginal herpes simplex type 2 (HSV-2) and Chlamydia trachomatis infection, and rectal HSV-2 infection.
In the vaginal HSV-2 challenge studies, 10% T-PSS gel provided significant protection against infection, even when administered 60 min prior to virus challenge (P < 0.0001). Both 5% and 10% T-PSS gel formulations significantly reduced the incidence of upper genital tract C. trachomatis infection in animals treated up to 5 min before challenge (P < 0.001). However, no protection against C. trachomatis infection was seen in animals treated 30 min before challenge. In mice challenged rectally with HSV-2, both the 5% and 10% T-PSS gels significantly reduced infection at 20 s (P < 0.01 for both). However, only the 10% gel provided significant protection when administered 5 min before challenge (P < 0.01).
T-PSS gel formulations have promising in vivo activity as topical microbicides.
研究聚（4-苯乙烯磺酸钠）（T-PSS）凝胶制剂作为局部杀菌剂的体内活性。
在2型单纯疱疹病毒（HSV-2）和沙眼衣原体阴道感染以及HSV-2直肠感染的小鼠模型中，检测凝胶制剂在病原体攻击前应用时降低感染发生率的能力。
在阴道HSV-2攻击研究中，即使在病毒攻击前60分钟给药，10% T-PSS凝胶也能提供显著的抗感染保护作用（P < 0.0001）。5%和10% T-PSS凝胶制剂在攻击前5分钟内给药的动物中，均显著降低了上生殖道沙眼衣原体感染的发生率（P < 0.001）。然而，在攻击前30分钟给药的动物中未观察到对沙眼衣原体感染的保护作用。在用HSV-2直肠攻击的小鼠中，5%和10% T-PSS凝胶在20秒时均显著降低了感染率（两者P均 < 0.01）。然而，只有10%凝胶在攻击前5分钟给药时提供了显著的保护作用（P < 0.01）。
T-PSS凝胶制剂作为局部杀菌剂具有良好的体内活性。
