Bózner P, Gombosová A, Valent M, Demes P, Alderete J F
Institute of Microbiology and Immunology, Medical Faculty, Comenius University, Bratislava, Czechoslovakia.
Parasitology. 1992 Dec;105 ( Pt 3):387-91. doi: 10.1017/s0031182000074552.
Immunoprecipitation combined with electrophoresis in gelatin-polyacrylamide gels was successfully used for detection of antibodies against numerous proteinases of Trichomonas vaginalis in infected patients. The method proved to be highly specific as anti-proteinase antibodies were absent in women with negative cultivation of T. vaginalis and no history of trichomoniasis. Sera of 71% and vaginal washes of 86% patients with trichomoniasis were positive for these antibodies. In vaginal washes, but not in sera, antibodies were partly complexed with proteinases, possibly of trichomonad origin. It was also shown that serum antibodies as well as local anti-proteinase antibodies persisted for weeks after patients had been cured.
免疫沉淀结合在明胶 - 聚丙烯酰胺凝胶中进行电泳，已成功用于检测感染患者体内针对阴道毛滴虫多种蛋白酶的抗体。该方法具有高度特异性，因为阴道毛滴虫培养阴性且无滴虫病病史的女性体内不存在抗蛋白酶抗体。71% 的滴虫病患者血清和86% 的患者阴道灌洗液中这些抗体呈阳性。在阴道灌洗液中，而非血清中，抗体部分与可能源自滴虫的蛋白酶形成复合物。还表明，患者治愈后，血清抗体以及局部抗蛋白酶抗体可持续存在数周。
