Alderete J F, Newton E, Dennis C, Neale K A
Department of Obstetrics and Gynecology, University of Texas Health Center, San Antonio 78284-7758.
Genitourin Med. 1991 Aug;67(4):331-4. doi: 10.1136/sti.67.4.331.
A recent report demonstrated the immunogenic character of the cysteine proteinases of Trichomonas vaginalis. It was of interest, therefore, to examine for the presence of serum anti-proteinase antibody among patients with trichomoniasis.
An immunoprecipitation assay was used involving protein A-bearing Staphylococcus aureus first coated with the IgG fraction of goat anti-human Ig and then mixed with individual sera of patients to bind human antibody. These antibody-coated bacteria were then added to detergent extracts of T vaginalis. Bound immune complexes on S aureus were washed and solubilised for electrophoretic analysis on acrylamide copolymerised with gelatin for detection of proteinase activity.
Sera from patients (50/50), but none from sera of normal, uninfected women, possessed IgG to numerous trichomonad cysteine proteinases. The presence of this serum anti-proteinase antibody disappeared after drug treatment and cure of patients of the T vaginalis infection.
The commonality of the anti-proteinase antibody in the sera of patients with trichomoniasis provided evidence for the expression of the same repertoire of parasite proteinases during infection. These observations have important implications for the in vivo relevance of the proteinases and indicate that strategies to use a specific serum antibody response for diagnosis of this infection may be possible.
最近的一份报告证实了阴道毛滴虫半胱氨酸蛋白酶的免疫原性。因此，研究滴虫病患者血清中抗蛋白酶抗体的存在情况很有意义。
采用免疫沉淀试验，先用山羊抗人IgG片段包被含蛋白A的金黄色葡萄球菌，然后与患者的个体血清混合以结合人抗体。然后将这些抗体包被的细菌加入阴道毛滴虫的去污剂提取物中。洗涤并溶解金黄色葡萄球菌上结合的免疫复合物，用于在与明胶共聚的丙烯酰胺上进行电泳分析，以检测蛋白酶活性。
患者血清（50/50）中存在针对多种滴虫半胱氨酸蛋白酶的IgG，而正常未感染女性的血清中则没有。在药物治疗和治愈患者的阴道毛滴虫感染后，这种血清抗蛋白酶抗体消失。
滴虫病患者血清中抗蛋白酶抗体的普遍性为感染期间寄生虫蛋白酶表达谱相同提供了证据。这些观察结果对蛋白酶在体内的相关性具有重要意义，并表明利用特异性血清抗体反应诊断这种感染的策略可能是可行的。
