Pawlik Andrzej, Florczak Marcin, Masiuk Marek, Machalinski Bogusław, Syczewska Marta, Szych Zbigniew, Gawrońska-Szklarz Barbara
Department of Pharmacokinetics and Therapeutic Drag Monitoring, Pomeranian University of Medicine, Szczecin, Poland.
Ann Transplant. 2003;8(2):54-6.
It has been observed that the organ manifestations in the patients with autoimmune diseases resulted from vessels damage may be caused by CD4+CD28- T lymphocytes, which express high levels of IFN gamma and posses cytolytic activity. The increased number of these cells in the recipients of allogenic bone marrow grafts and kidney grafts was also observed. The aim of the study was to evaluate the amount of CD4+CD28- T lymphocytes in the recipients of allogenic liver grafts. The size of CD4+CD28- T cells compartment has been determined in 20 patients after liver transplantation and 20 healthy subjects as control group using two-color FACS analysis. The frequency of CD4+CD28- T cells in the liver transplant recipient was 5.65% and was significantly higher than in control group 1.4% (p < 0.001). The increased level of cytotoxic CD4+CD28- T cells may be involved in vessels damage and gradual loss of graft function.
据观察，自身免疫性疾病患者中由血管损伤导致的器官表现可能由CD4+CD28-T淋巴细胞引起，这些细胞表达高水平的干扰素γ并具有细胞溶解活性。在同种异体骨髓移植和肾移植受者中也观察到这些细胞数量增加。本研究的目的是评估同种异体肝移植受者中CD4+CD28-T淋巴细胞的数量。采用双色荧光激活细胞分选分析，对20例肝移植患者和20例健康受试者作为对照组的CD4+CD28-T细胞区室大小进行了测定。肝移植受者中CD4+CD28-T细胞的频率为5.65%，显著高于对照组的1.4%（p<0.001）。细胞毒性CD4+CD28-T细胞水平的升高可能与血管损伤和移植物功能的逐渐丧失有关。
