Pawlik A, Florczak M, Masiuk M, Dutkiewicz G, Machalinski B, Rozanski J, Domanski L, Gawrońska-Szklarz B
Department of Pharmacokinetics and Therapeutic Drug Monitoring, Pomeranian University of Medicine, Al Powstańców Wielkopolskich 72, 70-111 Szczecin, Poland.
Transplant Proc. 2003 Dec;35(8):2902-4. doi: 10.1016/j.transproceed.2003.10.061.
CD4+CD28- T cells are oligoclonal lymphocytes rarely found in healthy subjects, but are present in high frequencies in patients with inflammatory diseases. Contrary to paradigm, they are functionally active and produce interferon gamma and cytolytic proteins, are cytotoxic in vessels and may contribute to tissue damage. The size of the peripheral blood CD4+CD28- T cell compartments was determined in 20 healthy individuals, 20 patients after renal transplantation with stable graft function, and 20 with chronic graft rejection by two-color FACS analysis. In patients with stable graft function, the median frequency of CD4+CD28- T cells was 3.1% and was significantly higher in comparison to the control group (1.4%) (P <.01). The highest subset CD4+CD28- cells was detected in patients with chronic graft rejection (10.65%). The amount of CD4+CD28- cells was significantly higher in this group in comparison to patients with stable graft function (P <.01). The evaluated number of CD4+CD28- cells in patients after renal transplantation, especially in graft recipients with chronic graft rejection, suggests a role of these cells in chronic graft destruction.
CD4+CD28- T细胞是寡克隆淋巴细胞，在健康受试者中很少见，但在炎症性疾病患者中高频存在。与传统观念相反，它们功能活跃，可产生γ干扰素和溶细胞蛋白，在血管中具有细胞毒性，并可能导致组织损伤。通过双色荧光激活细胞分选分析测定了20名健康个体、20名肾移植后移植功能稳定的患者以及20名慢性移植排斥患者外周血CD4+CD28- T细胞区室的大小。在移植功能稳定的患者中，CD4+CD28- T细胞的中位频率为3.1%，与对照组（1.4%）相比显著更高（P<.01）。在慢性移植排斥患者中检测到最高比例的CD4+CD28-细胞（10.65%）。与移植功能稳定的患者相比，该组中CD4+CD28-细胞的数量显著更高（P<.01）。肾移植患者，尤其是慢性移植排斥的移植受者中CD4+CD28-细胞的评估数量表明这些细胞在慢性移植破坏中起作用。
