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通过掺入二糖核苷实现DNA切割且不损失遗传信息。

Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside.

作者信息

Nauwelaerts Koen, Vastmans Karen, Froeyen Matheus, Kempeneers Veerle, Rozenski Jef, Rosemeyer Helmut, Van Aerschot Arthur, Busson Roger, Lacey Jeffrey C, Efimtseva Ekaterina, Mikhailov Sergey, Lescrinier Eveline, Herdewijn Piet

机构信息

Rega Institute for Medical Research, Laboratory for Medicinal Chemistry, Minderbroedersstraat 10, B-3000 Leuven, Belgium.

出版信息

Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. doi: 10.1093/nar/gkg911.

DOI:10.1093/nar/gkg911
PMID:14627809
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC290278/
Abstract

A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.

摘要

插入一个核苷酸的3'-羟基与下一个核苷酸的5'-磷酸基团之间的核糖残基，在DNA中作为一个位点特异性切割位点发挥作用。这个额外的核糖不会中断螺旋的形成，并且它保护双链DNA不被限制性酶切割。高碘酸盐可实现切割，氢氧化钠可去除所有核糖片段。由此，连接反应后可获得完整的天然寡脱氧核苷酸，这意味着位点特异性切割和完整DNA的回收在不损失遗传信息的情况下发生。

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