Jovicić Vilma Lj, Grego Edita M, Lako Branislav L, Ristović Blagoje M, Lepsanović Zorica A, Stajković Novica T
Institute of Public Health of Serbia Dr Milan Jovanović Batut, Department for Microbiology, Belgrade, Serbia and Montenegro.
APMIS. 2003 Nov;111(11):1053-9. doi: 10.1111/j.1600-0463.2003.apm1111107.x.
To improve the serodiagnosis of Lyme borreliosis (LB) the performances of four tests were evaluated. An indirect immunofluorescent assay based on Borrelia burgdorferi s.s., enzyme-linked immunosorbent assay (ELISA) based on local isolates of Borrelia afzelii and B. burgdorferi s.s., and immunoblot (IB) of B. afzelii were prepared. The serum panels contained 214 serum samples: control group (n=120) and patients at different stages of LB (n=94). The specificity of IB was 96%, of in-house ELISA 93%, and of IFA 89%. In early LB the sensitivity of IFA was 36%, ELISA 67%, and IB 93%. In late-stage LB the sensitivity was: 72% for IFA, 80% for ELISA, and 94% for IB. Comparison of in-house and Behring ELISA showed that the sensitivity of the serological assay could be increased when the test was based on local Borrelia strains. IgM and IgG antibodies from sera of patients with early and late LB most frequently demonstrated reactivity to OspC. The other significant proteins in early LB were: p39, p41 in IgM IB, and p83/100, p39, Osp17 in IgG IB; in late LB: p39, p41 in IgM IB, and p83/100, Osp17, p21 and p43 in IgG IB. Using IB based on local B. afzelii isolates improves the serodiagnosis of early LB in our geographical region.
为提高莱姆病（LB）的血清学诊断水平，对四项检测方法的性能进行了评估。制备了基于伯氏疏螺旋体狭义亚种的间接免疫荧光试验、基于阿氏疏螺旋体和伯氏疏螺旋体狭义亚种本地分离株的酶联免疫吸附试验（ELISA）以及阿氏疏螺旋体的免疫印迹（IB）。血清样本组包含214份血清样本：对照组（n = 120）和处于不同阶段的莱姆病患者（n = 94）。免疫印迹的特异性为96%，内部ELISA为93%，免疫荧光试验为89%。在莱姆病早期，免疫荧光试验的敏感性为36%，ELISA为67%，免疫印迹为93%。在莱姆病晚期，敏感性分别为：免疫荧光试验72%，ELISA 80%，免疫印迹94%。内部ELISA与贝林ELISA的比较表明，当检测基于本地疏螺旋体菌株时，血清学检测的敏感性可以提高。早期和晚期莱姆病患者血清中的IgM和IgG抗体最常显示对OspC有反应性。莱姆病早期其他重要蛋白为：IgM免疫印迹中的p39、p41，以及IgG免疫印迹中的p83/100、p39、Osp17；莱姆病晚期为：IgM免疫印迹中的p39、p41，以及IgG免疫印迹中的p83/100、Osp17、p21和p43。使用基于本地阿氏疏螺旋体分离株的免疫印迹可改善我们所在地理区域早期莱姆病的血清学诊断。
