Misl'anová Csilla, Hutta Milan
Institute of Preventive and Clinical Medicine, Limbová 14, SK-833 01 37 Bratislava, Slovak Republic.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Nov 25;797(1-2):91-109. doi: 10.1016/j.jchromb.2003.07.018.
The review article covers advances of chiral drugs analysis by high-performance liquid chromatography (HPLC) methods achieved during last 10 years. Emphasis is given to various aspects of influence of biological matrix in pharmacodynamics, pharmacokinetics, HPLC analysis. Discussed is composition of main biological matrices from the point of view of potential interferences to above-mentioned fields of study. Beside typical analytical approaches to chiral recognition in HPLC, sample pretreatment and/or clean-up by conventional extraction procedures, column switching (CSW) techniques using restricted access materials (RAMs), microdialysis (MCD) is discussed. Measurement of unbound drug concentration and discussion of column maintenance and remedy is an additional source of information and field where knowledge on complex properties and interactions of biological matrix is usefully applicable.
这篇综述文章涵盖了过去10年中通过高效液相色谱（HPLC）方法对手性药物分析所取得的进展。重点介绍了生物基质在药效学、药代动力学和HPLC分析中的各个影响方面。从对上述研究领域潜在干扰的角度讨论了主要生物基质的组成。除了HPLC中手性识别的典型分析方法、通过常规萃取程序进行的样品预处理和/或净化、使用限进材料（RAMs）的柱切换（CSW）技术外，还讨论了微透析（MCD）。游离药物浓度的测定以及柱维护和修复的讨论是另一个信息来源和领域，在其中关于生物基质复杂性质和相互作用的知识得到了有效应用。
