兔骨骼肌微透析液中3-甲氧基-4-羟基苯乙二醇的检测

Detection of 3-methoxy-4-hydroxyphenylglycol in rabbit skeletal muscle microdialysate.

作者信息

Tokunaga Noriyuki, Yamazaki Toji, Akiyama Tsuyoshi, Mori Hidezo

机构信息

Department of Cardiac Physiology, National Cardiovascular Center Research Institute, Fujishiro-dai 5-7-1, Suita, Osaka 565-8565, Japan.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Dec 5;798(1):163-6. doi: 10.1016/j.jchromb.2003.09.016.

DOI:10.1016/j.jchromb.2003.09.016

PMID:14630372

Abstract

A high-performance liquid chromatography with electrochemical detection (HPLC-ED) method is described for determination of 3-methoxy-4-hydroxyphenylglycol (MHPG) in microdialysate from the skeletal muscle interstitial space. Using a microdialysis technique, we sampled 30 microl dialysate from the skeletal muscle interstitial space and injected dialysate directly into HPLC-ED system. The control MHPG concentration of dialysate was 213+/-18 pg/ml. The MHPG concentrations were reduced by entacapone (catechol-O-methyltransferase inhibitor, COMT), augmented by local infusion of dihydroxyphenylglycol. This system offers a new possibility for simple, rapid monitoring of MHPG as an index of COMT activity in skeletal muscle.

摘要

描述了一种采用电化学检测的高效液相色谱法（HPLC-ED），用于测定骨骼肌间质空间微透析液中的3-甲氧基-4-羟基苯乙二醇（MHPG）。使用微透析技术，我们从骨骼肌间质空间采集了30微升透析液，并将透析液直接注入HPLC-ED系统。透析液中MHPG的对照浓度为213±18皮克/毫升。恩他卡朋（儿茶酚-O-甲基转移酶抑制剂，COMT）可降低MHPG浓度，而局部输注二羟基苯乙二醇可提高其浓度。该系统为简单、快速监测MHPG作为骨骼肌中COMT活性指标提供了新的可能性。