High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol. - Suppr | 超能文献

High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol.

作者信息

Takauchi Y, Kitagawa H, Kawada T, Akiyama T, Yamazaki T

机构信息

Department of Anesthesiology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):218-21. doi: 10.1016/s0378-4347(97)00003-0.

DOI:10.1016/s0378-4347(97)00003-0

Abstract

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-microl dialysates were sampled from the myocardial interstitial space (1-min fractions) and were injected directly into the HPLC-ED system. The in vitro quantification limit for DHPG was 250 fg in a 10-microl injection. The basal DHPG concentration of dialysate was 181+/-46 pg/ml. This system offers a new possibility for monitoring myocardial interstitial DHPG levels.

摘要

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