高效液相色谱法测定心肌间质二羟基苯乙二醇

High-performance liquid chromatographic determination of myocardial interstitial dihydroxyphenylglycol.

作者信息

Takauchi Y, Kitagawa H, Kawada T, Akiyama T, Yamazaki T

机构信息

Department of Anesthesiology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Japan.

出版信息

J Chromatogr B Biomed Sci Appl. 1997 May 23;693(1):218-21. doi: 10.1016/s0378-4347(97)00003-0.

DOI:10.1016/s0378-4347(97)00003-0

PMID:9200538

Abstract

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring dihydroxyphenylglycol (DHPG) in the myocardial interstitial space. Using a cardiac dialysis technique, 10-microl dialysates were sampled from the myocardial interstitial space (1-min fractions) and were injected directly into the HPLC-ED system. The in vitro quantification limit for DHPG was 250 fg in a 10-microl injection. The basal DHPG concentration of dialysate was 181+/-46 pg/ml. This system offers a new possibility for monitoring myocardial interstitial DHPG levels.

摘要

本研究描述了一种采用电化学检测的高效液相色谱法（HPLC-ED），用于监测心肌间质空间中的二羟基苯乙二醇（DHPG）。使用心脏透析技术，从心肌间质空间采集10微升透析液样本（1分钟的馏分），并直接注入HPLC-ED系统。在10微升进样量下，DHPG的体外定量限为250飞克。透析液的基础DHPG浓度为181±46皮克/毫升。该系统为监测心肌间质DHPG水平提供了新的可能性。