液相色谱法测定心肌间质肾上腺素

Liquid chromatographic determination of myocardial interstitial epinephrine.

作者信息

Kawada T, Yamazaki T, Akiyama T, Sato T, Shishido T, Sugimachi M, Inagaki M, Alexander J, Sunagawa K

机构信息

Department of Cardiovascular Dynamics, National Cardiovascular Center Research Institute, Suita, Osaka, Japan.

出版信息

J Chromatogr B Biomed Sci Appl. 1998 Sep 4;714(2):375-8. doi: 10.1016/s0378-4347(98)00221-7.

DOI:10.1016/s0378-4347(98)00221-7

PMID:9766879

Abstract

This study describes a high-performance liquid chromatographic method with electrochemical detection (HPLC-ED) for monitoring of epinephrine (Epi) in the myocardial interstitial space. The in vitro detection limit for Epi was 200 fg in a 50-microl injection. Using a cardiac dialysis technique, 60-microl dialysates were sampled from the myocardial interstitial space (6-min fractions). After an alumina procedure, the dialysate Epi concentration was measured using the HPLC-ED system. Although the basal Epi concentration was undetectable, local administration of desipramine increased Epi concentration of the dialysate to 38.1+/-18.5 pg/ml. This system affords a new possibility for estimating myocardial interstitial Epi level.

摘要

本研究描述了一种采用电化学检测的高效液相色谱法（HPLC-ED），用于监测心肌间质空间中的肾上腺素（Epi）。在50微升进样量下，Epi的体外检测限为200飞克。采用心脏透析技术，从心肌间质空间采集60微升透析液（6分钟的分样）。经过氧化铝处理后，使用HPLC-ED系统测量透析液中Epi的浓度。尽管基础Epi浓度无法检测到，但地昔帕明的局部给药使透析液中Epi浓度升高至38.1±18.5皮克/毫升。该系统为估计心肌间质Epi水平提供了新的可能性。