Fernandez-Garcia Nieves, Carvajal Micaela, Olmos Enrique
Departamento de Fisiología y Nutrición Vegetal, Centro de Edafología y Biología Aplicada del Segura, CSIC, Aptdo. Correos 164, 30100 Murcia, Spain.
Ann Bot. 2004 Jan;93(1):53-60. doi: 10.1093/aob/mch014. Epub 2003 Nov 20.
The use of grafted plants in vegetable crop production is now being expanded greatly. However, few data are available on the formation of graft unions in vegetables. In this work, the structural development of the graft union formation in tomato plants is studied, together with the possible relationship with activities of peroxidases and catalases.
Tomato (Lycopersicon esculentum Mill.) seedlings of cultivar Fanny were grafted on the rootstock of cultivar AR-9704 using the 'tongue approach grafting' method, and were grown in a crop chamber. A study of the structural development of the graft union and the involvement of peroxidases and catalases in the process of graft formation was carried out during the first stages of the graft union (4, 8 and 15 d after grafting).
Observation of the structure of the graft union showed formation of xylem and phloem vessels through the graft union 8 d after grafting. In addition, root hydraulic conductance, L0, indicate that the graft union is fully functional 8 d after grafting, which coincided with an increase of peroxidase and catalase activities.
These results suggest that increased peroxidase and catalase activities might be implicated in graft development in tomato plants.
嫁接植物在蔬菜作物生产中的应用目前正在大幅扩展。然而，关于蔬菜中嫁接结合部形成的数据却很少。在这项研究中，对番茄植株嫁接结合部的结构发育进行了研究，并探讨了其与过氧化物酶和过氧化氢酶活性之间可能存在的关系。
采用“舌接”法将番茄品种Fanny的幼苗嫁接到品种AR - 9704的砧木上，并在作物培养室内生长。在嫁接结合部形成的最初阶段（嫁接后4、8和15天），对嫁接结合部的结构发育以及过氧化物酶和过氧化氢酶在嫁接形成过程中的作用进行了研究。
对嫁接结合部结构的观察表明，嫁接后8天通过嫁接结合部形成了木质部和韧皮部导管。此外，根系水力导度L0表明，嫁接后8天嫁接结合部已完全发挥功能，这与过氧化物酶和过氧化氢酶活性的增加相吻合。
这些结果表明，过氧化物酶和过氧化氢酶活性的增加可能与番茄植株的嫁接发育有关。
