哺乳动物端粒酶中的模板边界定义
Template boundary definition in mammalian telomerase.
作者信息
Chen Jiunn-Liang, Greider Carol W
机构信息
Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
出版信息
Genes Dev. 2003 Nov 15;17(22):2747-52. doi: 10.1101/gad.1140303.
Abstract
Telomerase uses a short template sequence in its intrinsic RNA component to synthesize telomere repeats. Disruption of the helix P1b in human telomerase RNA or alteration of its distance from the template resulted in telomerase copying residues past the normal template boundary both in vivo and in vitro. Therefore, helix P1b is important for template boundary definition in human telomerase. Mouse telomerase RNA lacks helix P1b, and the boundary is established at 2 nt downstream of the 5'-end. The divergent structure of boundary definition elements in mammals, yeast, and ciliates suggests diverse mechanisms for template boundary definition in telomerase.
摘要
端粒酶利用其内在RNA组分中的短模板序列来合成端粒重复序列。人端粒酶RNA中螺旋P1b的破坏或其与模板距离的改变导致端粒酶在体内和体外都复制超过正常模板边界的残基。因此，螺旋P1b对人端粒酶中模板边界的定义很重要。小鼠端粒酶RNA缺乏螺旋P1b，其边界在5'端下游2个核苷酸处确定。哺乳动物、酵母和纤毛虫中端粒酶模板边界定义元件的不同结构表明端粒酶中模板边界定义存在多种机制。
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