Minihan D, O'Mahony M, Whyte P, Collins J D
Department of Agriculture and Food, Central Research Veterinary Laboratory, Abbotstown, Castleknock, Dublin 15, Ireland.
J Vet Med B Infect Dis Vet Public Health. 2003 Oct;50(8):378-82. doi: 10.1046/j.1439-0450.2003.00674.x.
The aim of the study was to investigate the effect of transport and lairage on the prevalence of Escherichia coli O157 faecal shedding and the subsequent contamination of beef carcasses. Individual rectal faecal samples were taken from two cohorts of cattle (109 and 59) at the farm before transport and at the abattoir post-transport and lairage. The entire outer and inner surfaces of the carcass of each animal were swabbed immediately following slaughter and dressing. The prevalence of E. coli O157 shedding in cattle sampled at farm, post-transport and lairage was 18% (20), 13% (14) and 12% (13) for cohort A and 1.7% (1), 1.7% (1) and 0 for cohort B, respectively. No E. coli O157 was recovered from the 168 dressed carcasses. In total, 98% (46 of 47) of the E. coli O157 isolates from cohort A were potentially pathogenic to man. Transport and lairage do not cause an increase in the prevalence of E. coli O157 faecal shedding in cattle. This study demonstrates that even positive cohorts of cattle may be slaughtered and processed to produce clean carcasses by following good hygienic practices.
本研究的目的是调查运输和圈养对大肠杆菌O157粪便排泄率以及随后牛肉胴体污染情况的影响。在运输前于农场以及运输和圈养后在屠宰场，从两组牛群（分别为109头和59头）中采集个体直肠粪便样本。每头动物屠宰和修整后，立即对其胴体的整个内外表面进行擦拭取样。A组在农场、运输后和圈养后采样的牛中，大肠杆菌O157的排泄率分别为18%（20头）、13%（14头）和12%（13头）；B组分别为1.7%（1头）、1.7%（1头）和0。在168具修整后的胴体中未检测到大肠杆菌O157。总体而言，A组分离出的大肠杆菌O157菌株中，98%（47株中的46株）对人类具有潜在致病性。运输和圈养不会导致牛群中大肠杆菌O157粪便排泄率增加。本研究表明，即使是携带大肠杆菌O157的牛群，通过遵循良好的卫生规范，也可以进行屠宰和加工，从而生产出清洁的胴体。
