Herrmann A, Korte T, Arnold K, Hillebrecht B
Institut für Biophysik, Fachbereich Biologie, Humboldt-Universität Berlin, Germany.
Antiviral Res. 1992 Oct 1;19(4):295-311. doi: 10.1016/0166-3542(92)90011-s.
Dextran sulfate suppresses the low pH-induced fusion of influenza virus A/Brazil 11/78 with erythrocyte membranes, as shown by fluorescence dequenching assay, using the fluorophore octadecylrhodamine B chloride (R18). Inhibition of fusion was maximal at pH 5.0, while at higher pH values (> 5.6) fusion was not affected. Hemolysis of intact red blood cells by influenza A virus at low pH values is also prevented by dextran sulfate. The inhibiting effect of the polymer is mainly ascribed to repression of virus attachment. Evidence is given that the conformational change of the virus envelope protein hemagglutinin (HA) responsible for triggering fusion is not affected by the polymer.
硫酸葡聚糖可抑制低pH诱导的甲型/巴西11/78流感病毒与红细胞膜的融合，如荧光猝灭试验所示，该试验使用了荧光团十八烷基罗丹明B氯化物（R18）。在pH 5.0时融合抑制作用最大，而在较高pH值（>5.6）时融合不受影响。硫酸葡聚糖还可防止甲型流感病毒在低pH值下对完整红细胞的溶血作用。该聚合物的抑制作用主要归因于对病毒附着的抑制。有证据表明，负责触发融合的病毒包膜蛋白血凝素（HA）的构象变化不受该聚合物影响。
