Torres Carmen, Tenorio Carmen, Portillo Aránzazu, García Maite, Martínez Carolina, Del Campo Rosa, Ruiz-Larrea Fernanda, Zarazaga Myriam
Area Bioquímica y Biología Molecular, Universidad de La Rioja, Logroño, Spain.
Microb Drug Resist. 2003;9 Suppl 1:S47-52. doi: 10.1089/107662903322541892.
Fecal samples of healthy animals (66 pigs, 22 pets) recovered during 1998 in La Rioja, Spain, were analyzed for vancomycin-resistant enterococci colonization. Vancomycin resistance mechanisms were analyzed by PCR and sequencing. vanA-containing enterococci were detected in 3 of 66 samples (4.5%) and 5 of 22 samples (22.7%) of the pig and pet samples, respectively. Seven unrelated pulsed-field gel electrophoresis (PFGE) patterns were detected among the 8 vanA isolates (7 Enterococcus faecium, 1 E. faecalis). The tet(M) gene was present in all eight vanA enterococcal isolates, while the erm(B) and aac(6')-Ie-aph(2")-Ia genes were detected in 6 and 3 isolates, respectively. Colonization by vanC-1-containing enterococci (E. gallinarum) was demonstrated in 3% and 4.5% of the pig and pet samples. The aac(6')-Ie-aph(2")-Ia, ant(6)-Ia, aph(3')-IIIa, erm(B) and tet(M) genes were identified in one of the E. gallinarum isolates from a pig fecal sample. One vanB2-containing E. hirae strain was detected in the fecal sample of a healthy pig. In this isolate, the vanB2 gene cluster was integrated into the Tn5382-like element, as demonstrated by specific PCRs and sequencing. The tet(M) and erm(B) genes were also detected in this isolate. This is the first report in which a vanB2-containing enterococci is detected in animals and in E. hirae.
对1998年在西班牙拉里奥哈采集的健康动物（66头猪、22只宠物）的粪便样本进行了耐万古霉素肠球菌定植分析。通过聚合酶链反应（PCR）和测序分析了万古霉素耐药机制。在猪和宠物样本中，分别在66份样本中的3份（4.5%）和22份样本中的5份（22.7%）检测到含vanA的肠球菌。在8株vanA分离株（7株粪肠球菌、1株屎肠球菌）中检测到7种不相关的脉冲场凝胶电泳（PFGE）图谱。所有8株含vanA的肠球菌分离株均存在tet(M)基因，而erm(B)基因和aac(6')-Ie-aph(2")-Ia基因分别在6株和3株分离株中检测到。在3%的猪样本和4.5%的宠物样本中证实了含vanC-1的肠球菌（鹑鸡肠球菌）的定植。在一份猪粪便样本的一株鹑鸡肠球菌分离株中鉴定出aac(6')-Ie-aph(2")-Ia、ant(6)-Ia、aph(3')-IIIa、erm(B)和tet(M)基因。在一头健康猪的粪便样本中检测到一株含vanB2的希拉肠球菌菌株。通过特异性PCR和测序证明，在该分离株中，vanB2基因簇整合到了Tn5382样元件中。在该分离株中也检测到了tet(M)基因和erm(B)基因。这是首次在动物和希拉肠球菌中检测到含vanB2的肠球菌的报告。
