Rosas-Acevedo José Luis, Boucias Drion G, Lezama Roberto, Sims Kelly, Pescador Alfonso
Centro de Desarrollo Regional, Unidad de Estudios de Posgrado e Investigación de Acapulco, Universidad Autónoma de Guerrero, Apdo. Postal 612, 39301 Acapulco, Gro., Mexico.
Exp Appl Acarol. 2003;29(3-4):213-25. doi: 10.1023/a:1025801817004.
The acaricidal mycopathogen Hirsutella thompsonii has been found to secrete metabolites that are active against female Tetranychus urticae. Specifically, the rose-colored exudate produced on sporulating cultures of Mexican HtM120I strain sterilized female spider mites in a dose-dependent fashion. Topical application of the exudate resulted in a 100% reduction in mite fecundity over the initial six days of experimentation. Depending upon the exudate dosage, mites partially recovered within 3 and 6 d post-treatment and produced a limited number of eggs. The spider mite active HtM120I exudate contained less detectable HtA toxin than the HtM120I broth filtrate, and it was innocuous when injected into the greater wax moth Galleria mellonella L. larvae. Broth filtrates of HtM120I cultures, although toxic to assayed G. mellonella larvae, did not inhibit mite oviposition to the degree or duration of the exudate preparations. These findings suggest that the factor responsible for suppressing oviposition in female spider mites is linked to the sporulation process and is distinct from the well-characterized HtA produced by vegetative cells.
杀螨病原真菌汤普森被毛孢已被发现能分泌对雌性二斑叶螨有活性的代谢产物。具体而言，墨西哥HtM120I菌株产孢培养物上产生的玫瑰色渗出物能以剂量依赖的方式使雌性叶螨绝育。在实验的最初六天，局部施用渗出物导致叶螨繁殖力下降100%。根据渗出物剂量的不同，叶螨在处理后3天和6天内部分恢复，并产下数量有限的卵。与HtM120I肉汤滤液相比，具有杀螨活性的HtM120I渗出物中可检测到的HtA毒素较少，并且将其注射到大蜡螟幼虫体内时是无害的。HtM120I培养物的肉汤滤液虽然对测定的大蜡螟幼虫有毒，但对叶螨产卵的抑制程度和持续时间不如渗出物制剂。这些发现表明，抑制雌性叶螨产卵的因素与产孢过程有关，并且与营养细胞产生的特征明确的HtA不同。
