El Tamer Mazen K, Smeets Monique, Holthuysen Nancy, Lücker Joost, Tang Ameing, Roozen Jacques, Bouwmeester Harro J, Voragen Alphons G J
Wageningen-UR, Plant Research International, 6700 AA Wageningen, The Netherlands.
J Biotechnol. 2003 Dec 5;106(1):15-21. doi: 10.1016/j.jbiotec.2003.09.003.
Monoterpenes are an important class of terpenoids that are commonly present in plant essential oils. These can be extracted from plants and are used in the flavouring and perfumery industry. Monoterpene synthases are the key enzymes in monoterpene biosynthesis, as they catalyse the cyclisation of the ubiquitous geranyl diphosphate (GDP) to the specific monoterpene skeletons. Tobacco is one of the most studied model plants, it can easily and efficiently be transformed, and is a suitable model to study the release of plant volatiles. Thus, we have isolated monoterpene synthases from lemon, transformed tobacco with these cDNAs and have used human panelists to study the change in fragrance of the transgenic in comparison to the wild type plants. In a triangle test, we found that subjects were capable of smelling significant differences between leaf samples. However, as a result of variability in panel ratings, no significant difference between two sets of transgenic flowers and the wild type tobacco flowers was found for the generated attributes in a descriptive test.
单萜是一类重要的萜类化合物，常见于植物精油中。它们可以从植物中提取出来，并用于调味和香料工业。单萜合酶是单萜生物合成中的关键酶，因为它们催化普遍存在的香叶基二磷酸（GDP）环化形成特定的单萜骨架。烟草是研究最多的模式植物之一，它能够轻松高效地进行转化，是研究植物挥发物释放的合适模式。因此，我们从柠檬中分离出单萜合酶，用这些cDNA转化烟草，并使用人类评判员来研究转基因植物与野生型植物相比香味的变化。在三角试验中，我们发现受试者能够嗅出叶片样本之间的显著差异。然而，由于评判员评分存在变异性，在描述性试验中，两组转基因花朵与野生型烟草花朵在所产生的属性方面未发现显著差异。
