Muñoz-Bertomeu Jesús, Ros Roc, Arrillaga Isabel, Segura Juan
Departamento de Biología Vegetal, Facultad de Farmacia, Universidad de Valencia, 46100 Burjasot, Valencia, Spain.
Metab Eng. 2008 May-Jul;10(3-4):166-77. doi: 10.1016/j.ymben.2008.04.002. Epub 2008 May 3.
We generated transgenic spike lavender (Lavandula latifolia) plants constitutively expressing the limonene synthase (LS) gene from spearmint (Mentha spicata), encoding the LS enzyme that catalyzes the synthesis of limonene from geranyl diphosphate. Overexpression of the LS transgene did not consistently affect monoterpene profile in pooled leaves or flowers from transgenic T(0) plants. Analyses from cohorts of leaves sampled at different developmental stages showed that essential oil accumulation in transgenic and control plants was higher in developing than in mature leaves. Furthermore, developing leaves of transgenic plants contained increased limonene contents (more than 450% increase compared to controls) that correlated with the highest transcript accumulation of the LS gene. The levels of other monoterpene pathway components were also significantly altered. T(0) transgenic plants were grown for 2 years, self-pollinated, and the T(1) seeds obtained. The increased limonene phenotype was maintained in the progenies that inherited the LS transgene.
我们培育了组成型表达留兰香（Mentha spicata）柠檬烯合酶（LS）基因的转基因穗花薰衣草（Lavandula latifolia）植株，该基因编码催化香叶基二磷酸合成柠檬烯的LS酶。LS转基因的过表达并未始终如一地影响转基因T(0)植株的叶片或花朵混合样本中的单萜类物质谱。对不同发育阶段采集的叶片样本队列进行的分析表明，转基因植株和对照植株中精油在发育中的叶片中的积累量高于成熟叶片。此外，转基因植株的发育叶片中柠檬烯含量增加（与对照相比增加超过450%），这与LS基因的最高转录本积累相关。其他单萜类途径成分的水平也发生了显著变化。T(0)转基因植株种植了2年，进行自花授粉，并获得了T(1)种子。继承了LS转基因的后代中维持了柠檬烯增加的表型。
