利用来自柠檬的三种单萜合酶进行代谢工程后，烟草植株香气增加且发生改变。
Increased and altered fragrance of tobacco plants after metabolic engineering using three monoterpene synthases from lemon.
作者信息
Lücker Joost, Schwab Wilfried, van Hautum Bianca, Blaas Jan, van der Plas Linus H W, Bouwmeester Harro J, Verhoeven Harrie A
机构信息
Business Unit Bioscience, Plant Research International, P.O. Box 16, 6700 AA, Wageningen, The Netherlands.
出版信息
Plant Physiol. 2004 Jan;134(1):510-9. doi: 10.1104/pp.103.030189. Epub 2004 Jan 8.
Abstract
Wild-type tobacco (Nicotiana tabacum) plants emit low levels of terpenoids, particularly from the flowers. By genetic modification of tobacco cv Petit Havana SR1 using three different monoterpene synthases from lemon (Citrus limon L. Burm. f.) and the subsequent combination of these three into one plant by crossings, we show that it is possible to increase the amount and alter the composition of the blend of monoterpenoids produced in tobacco plants. The transgenic tobacco plant line with the three introduced monoterpene synthases is emitting beta-pinene, limonene, and gamma-terpinene and a number of side products of the introduced monoterpene synthases, from its leaves and flowers, in addition to the terpenoids emitted by wild-type plants. The results show that there is a sufficiently high level of substrate accessible for the introduced enzymes.
摘要
野生型烟草（Nicotiana tabacum）植株会释放低水平的萜类化合物，尤其是从花朵中释放。通过使用来自柠檬（Citrus limon L. Burm. f.）的三种不同单萜合酶对烟草品种 Petit Havana SR1 进行基因改造，并随后通过杂交将这三种酶组合到一株植物中，我们表明可以增加烟草植株中产生的单萜类化合物混合物的数量并改变其组成。具有三种导入单萜合酶的转基因烟草株系，除了野生型植株释放的萜类化合物外，还从其叶片和花朵中释放β-蒎烯、柠檬烯、γ-萜品烯以及导入单萜合酶的一些副产物。结果表明，对于导入的酶而言，有足够高水平的底物可供利用。
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