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通过在烟草细胞中表达黑曲霉果胶甲酯酶可提高甲醇产量。

Methanol production is enhanced by expression of an Aspergillus niger pectin methylesterase in tobacco cells.

作者信息

Hasunuma Tomohisa, Fukusaki Ei-ichiro, Kobayashi Akio

机构信息

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1, Yamadaoka, Suita 565-0871, Japan.

出版信息

J Biotechnol. 2003 Dec 5;106(1):45-52. doi: 10.1016/j.jbiotec.2003.07.008.

DOI:10.1016/j.jbiotec.2003.07.008
PMID:14636709
Abstract

Tobacco suspension culture cell (Nicotiana tabacum, BY2) was transformed with an Aspergillus niger pectin methylesterase (PME; EC 3.1.1.11) cDNA under the control of cauliflower mosaic virus (CaMV) 35S promoter. The transformant indicated a significant rise of PME and the level of methanol in the transformant increased by 28.7% compared to the vector control transformant. This is the first report of methanol overproduction in plant cells by means of genetic engineering.

摘要

用受花椰菜花叶病毒（CaMV）35S启动子控制的黑曲霉果胶甲酯酶（PME；EC 3.1.1.11）cDNA转化烟草悬浮培养细胞（烟草，BY2）。与载体对照转化体相比，转化体的PME显著升高，转化体中的甲醇水平增加了28.7%。这是通过基因工程在植物细胞中过量生产甲醇的首次报道。

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