Komarova T V, Pozdyshev D V, Petrunia I V, Sheshukova E V, Dorokhov Y L
Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Biochemistry (Mosc). 2014 Feb;79(2):102-10. doi: 10.1134/S0006297914020035.
Plant leaves undergo a sink-source modification of intercellular macromolecular transport during the transition from carbon import to carbon export. After assessing the role of metabolite signaling in gene regulation in Nicotiana tabacum sink and source leaves, we observed increased pectin methylesterase (PME)-mediated methanol generation in immature leaves. Using suppression subtractive hybridization (SSH), we identified a number of genes whose activity changes from sink to source leaves. The most abundant SSH-identified genes appeared to be sensitive to methanol. We hypothesize that tobacco leaf maturation and the sink-source transition are accompanied by a change in mRNA levels of genes that function in methanol-dependent cell signaling.
在从碳输入向碳输出的转变过程中，植物叶片的细胞间大分子运输会经历从库到源的转变。在评估了代谢物信号传导在烟草库叶和源叶基因调控中的作用后，我们观察到未成熟叶片中果胶甲酯酶（PME）介导的甲醇生成增加。利用抑制性消减杂交（SSH）技术，我们鉴定出了一些活性从库叶向源叶发生变化的基因。SSH鉴定出的最丰富的基因似乎对甲醇敏感。我们推测，烟草叶片的成熟和库源转变伴随着在甲醇依赖性细胞信号传导中起作用的基因的mRNA水平的变化。
