State Key Laboratory of Cotton Biology, Key Laboratory of Biological and Genetic Breeding of Cotton, the Ministry of Agriculture, Institute of Cotton Research, Chinese Academy of Agricultural Science, Anyang, Henan, China.
Institute of Molecular Biology and Biotechnology, the University of Lahore, Lahore-Pakistan.
GM Crops Food. 2021 Jan 2;12(1):382-395. doi: 10.1080/21645698.2021.1944013.
The idea of enhanced methanol production from cell wall by pectin methyl esterase enzymes (PME) combined with expression of genes from as a strategy to improve insect pest control in cotton is presented. We constructed a cassette containing two genes ( and ) and two genes, one from (), and other from () in pCAMBIA1301 plant expression vector using CAMV-35S promoter. This construction was transformed in Eagle-2 cotton variety by using shoot apex-cut -mediated transformation. Expression of genes and genes was confirmed by qPCR. Methanol production was measured in control and in the and transformed plants showing methanol production only in transformed plants, in contrast to the non-transgenic cotton plants. Finally, insect bioassays performed with transgenic plants expressing and genes showed 100% mortality for (cotton bollworm) larvae, 70% mortality for (pink bollworm) larvae and 95% mortality of , (spotted bollworm) larvae, that was higher than the transgenic plants expressing only genes that showed 84%, 49% and 79% mortality, respectively. These results demonstrate that Bt. -genes coupled with genes are an effective strategy to improve the control of different insect pests.
提出了通过果胶甲酯酶（PME）增强细胞壁中甲醇生产，结合表达 基因作为提高棉花害虫防治的策略。我们构建了一个包含两个 基因（和）和两个 基因的盒，一个来自 （），另一个来自 （），在 pCAMBIA1301 植物表达载体中使用 CAMV-35S 启动子。通过茎尖切割介导转化将该构建体转化到 Eagle-2 棉花品种中。通过 qPCR 证实了 基因和 基因的表达。在对照和 及 转化的植物中测量甲醇的产生，仅在转化的植物中检测到甲醇的产生，而在非转基因棉花植物中则没有。最后，用表达 基因和 基因的转基因植物进行昆虫生物测定，结果表明 （棉铃虫）幼虫的死亡率为 100%， （粉斑螟）幼虫的死亡率为 70%， 和 （点斑螟）幼虫的死亡率为 95%，高于仅表达 基因的转基因植物，其死亡率分别为 84%、49%和 79%。这些结果表明，Bt. -基因与 基因的结合是提高控制不同害虫的有效策略。
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