Lukkari P, Jumppanen J, Jinno K, Elo H, Riekkola M L
University of Helsinki, Department of Chemistry, Finland.
J Pharm Biomed Anal. 1992 Aug;10(8):561-5. doi: 10.1016/0731-7085(92)80081-w.
A micellar electrokinetic capillary chromatography method was developed for the separation and determination of aliphatic congeners of bis(amidinohydrazones) in standard solution. Eight bis(amidinohydrazones) could be determined in less than 15 min at an applied voltage of 22 kV, using 0.05 M sodium phosphate as buffer (pH 7.0) together with 1 mM N-cetyl-N,N,N-trimethylammonium bromide. Hydrostatic sample injection was employed. The method exhibited good repeatability and a linear range of 2.5-100 micrograms ml-1. A detection limit of 1 micrograms ml-1 was achieved. The method also allows the determination of bis(amidinohydrazones) in human serum samples.
建立了一种胶束电动毛细管色谱法，用于分离和测定标准溶液中双（脒腙）的脂肪族同系物。在22 kV的施加电压下，使用0.05 M磷酸钠作为缓冲液（pH 7.0）和1 mM溴化十六烷基三甲基铵，可在不到15分钟内测定8种双（脒腙）。采用静水压进样。该方法具有良好的重复性，线性范围为2.5 - 100微克/毫升，检测限为1微克/毫升。该方法还可用于测定人血清样品中的双（脒腙）。
