Palfreyman John W, Gartland Jill S, Sturrock Craig J, Lester Doug, White Nia A, Low Gordon A, Bech-Andersen Joergen, Cooke David E L
SIMBIOS, University of Abertay Dundee, Bell Street, Dundee DD1 1HG, UK.
FEMS Microbiol Lett. 2003 Nov 21;228(2):281-6. doi: 10.1016/S0378-1097(03)00783-3.
Molecular and morphological parameters of Serpula lacrymans isolates from various sites in the built environment in Europe and Australia were compared to similar parameters of 'wild' isolates from India, the Sumava Mountains (Czech Republic) and Mount Shasta (USA). The Indian, Czech Republic and all of the building isolates bar one showed identity in both molecular and morphological features. The Australian and the USA isolates (BF-050 and USA'94 respectively) showed specific morphological differences and could be separated on the basis of randomly amplified polymorphic deoxyribonucleic acid polymerase chain reaction (RAPD PCR) with the USA isolate being least closely related to the S. lacrymans type strain of FPRL12C. ITS sequence data revealed two base differences between FPRL12C and BF-050 in the 673 sequenced, nine differences between FPRL12C and USA'94 and 16 differences between USA'94 and the closely related organism Serpula himantioides. The possible evolutionary relationships between the various isolates are discussed along with suggestions for the origin of S. lacrymans as a scourge of the built environment in many temperate areas of the world.
将来自欧洲和澳大利亚建筑环境中不同地点的桑黄拟层孔菌分离株的分子和形态学参数，与来自印度、苏马瓦山脉（捷克共和国）和沙斯塔山（美国）的“野生”分离株的类似参数进行了比较。印度、捷克共和国的所有建筑分离株以及除一个之外的所有建筑分离株在分子和形态特征上都表现出一致性。澳大利亚和美国的分离株（分别为BF - 050和USA'94）表现出特定的形态差异，并且可以基于随机扩增多态性脱氧核糖核酸聚合酶链反应（RAPD PCR）进行区分，其中美国分离株与FPRL12C的桑黄拟层孔菌模式菌株关系最不密切。ITS序列数据显示，在测序的673个碱基中，FPRL12C与BF - 050之间有两个碱基差异，FPRL12C与USA'94之间有九个差异，USA'94与密切相关的物种桑黄之间有16个差异。讨论了各种分离株之间可能的进化关系，并对桑黄拟层孔菌作为世界许多温带地区建筑环境祸害的起源提出了建议。
