Enyaru J C, Odiit M, Gashumba J K, Carasco J F, Rwendeire A J
Uganda Trypanosomiasis Research Organization (UTRO), Tororo, Uganda.
Bull World Health Organ. 1992;70(5):631-6.
An epidemic of sleeping sickness, which started in 1976 in a focus within the county of Luuka in Central Busoga, has spread to cover the three districts of Busoga and large parts of the neighbouring districts of Tororo and Mukono. Forty-three isolates of the subgenus Trypanozoon from Busoga and Tororo (27 from man, 9 from cows, 2 from pigs and 5 from tsetse flies) were compared by thin-layer starch-gel electrophoresis for seven enzymes. Thirty zymodemes were identified; 17 of them were found circulating in the human population. The zymodemes seen previously in Busoga were still circulating together with several new ones. Of the 16 isolates from cattle, pigs and tsetse flies, only two had the same profile, indicating a high degree of diversity. Two zymodemes from cows and a pig were identical to those found in man, implicating domestic stock in the transmission of human disease in south-east Uganda. A computer analysis of the results produced six main zymodeme groups. One comprised only isolates from man; two were composed of isolates from man, domestic animals and tsetse; and three consisted of stocks from domestic animals only. These groups quite probably indicate the different cycles of transmission involving man, tsetse fly and domestic stock.
昏睡病疫情于1976年在中布索加卢卡县的一个疫源地爆发，现已蔓延至布索加的三个区以及邻近的托罗罗区和穆科诺区的大部分地区。对来自布索加和托罗罗的43株锥虫亚属分离株（27株来自人，9株来自牛，2株来自猪，5株来自采采蝇）进行了七种酶的薄层淀粉凝胶电泳比较。共鉴定出30种酶型；其中17种在人群中传播。以前在布索加发现的酶型仍与几种新的酶型一起传播。在从牛、猪和采采蝇中分离出的16株菌株中，只有两株具有相同的酶谱，这表明多样性程度很高。来自牛和猪的两种酶型与在人类中发现的酶型相同，这表明家畜在乌干达东南部人类疾病传播中起到了作用。对结果进行的计算机分析产生了六个主要的酶型组。一组仅包括来自人类的分离株；两组由来自人类、家畜和采采蝇的分离株组成；三组仅由家畜的菌株组成。这些组很可能表明涉及人类、采采蝇和家畜的不同传播周期。
