Otieno L H, Darji N
International Centre of Insect Physiology and Ecology (ICIPE), Nairobi, Kenya.
J Trop Med Hyg. 1987 Oct;90(5):259-63.
During studies to determine the main Trypanosoma brucei rhodesiense transmission sites in Lambwe Valley, Western Kenya, Glossina pallidipes were collected from two areas in the valley and examined for trypanosome infection. T. brucei isolated from infected flies were tested for their response to the lethal effects of human blood (Blood Incubation Infectivity Test, BIIT) and also characterized using isoenzyme electrophoresis. Six of the 26 T. brucei tested were BIIT positive, two of which had enzyme profiles identical to human isolates. The 26 isolates were grouped into 10 zymodemes. Two zymodemes were identical to T. b. rhodesiense isolated from sleeping sickness patients, one of which was identical to the predominant zymodemes among patients from Lambwe Valley area. The other zymodeme was identical to an isolate from a patient from the Busoga (Uganda) sleeping sickness epidemic focus. These two Zymodemes were BIIT positive. It is suggested that there has been an exchange of human infecting organisms between the Kenya and the Uganda foci.
在确定肯尼亚西部兰贝谷布氏罗得西亚锥虫主要传播地点的研究过程中，从该山谷的两个区域采集了淡足舌蝇，并检查其锥虫感染情况。从受感染的苍蝇中分离出布氏锥虫，检测其对人血致死效应的反应（血液培养感染性试验，BIIT），并通过同工酶电泳进行特征分析。在检测的26株布氏锥虫中，有6株BIIT呈阳性，其中2株的酶谱与从人类分离株相同。这26株分离株被分为10个酶带型。有两个酶带型与从昏睡病患者分离出的布氏罗得西亚锥虫相同，其中一个与兰贝谷地区患者中的主要酶带型相同。另一个酶带型与来自布索加（乌干达）昏睡病流行区一名患者的分离株相同。这两个酶带型BIIT呈阳性。表明在肯尼亚和乌干达疫源地之间存在人类感染性生物体的交换。
