England L S, Gorzelak M, Trevors J T
Laboratory of Microbial Technology, Department of Environmental Biology, University of Guelph, Guelph, ON, Canada N1G 2W1.
Biochim Biophys Acta. 2003 Dec 5;1624(1-3):76-80. doi: 10.1016/j.bbagen.2003.09.012.
Growth and membrane polarization of Pseudomonas aeruginosa UG2 cells grown under randomized microgravity (RMG) and 1xg were measured in a high aspect ratio vessel (HARV) and also in batch cultures mixed at 12 and 150 rpm in Erlenmeyer shake flasks. Membrane polarization was measured using the fluorescent probe, 1,6-diphenyl-1,3,5-hexatriene (DPH). No differences were observed in the growth curves or membrane polarization values (about 0.300) under all three culture conditions. However, the net effect of RMG at the single cell level may be still unknown. It may be possible that RMG effects are species-dependent or bacterial cells with a small mass and volume may be near the threshold where RMG exerts a minimal effect.
在高长宽比容器（HARV）中以及在锥形瓶摇瓶中以12和150 rpm混合的分批培养物中，测量了在随机微重力（RMG）和1xg条件下生长的铜绿假单胞菌UG2细胞的生长和膜极化。使用荧光探针1,6-二苯基-1,3,5-己三烯（DPH）测量膜极化。在所有三种培养条件下，生长曲线或膜极化值（约0.300）均未观察到差异。然而，RMG在单细胞水平上的净效应可能仍然未知。RMG效应可能取决于物种，或者质量和体积较小的细菌细胞可能接近RMG发挥最小作用的阈值。
