Reitz M, DasGupta K, Brandt L
Department of Physiological Chemistry, Duesbergweg, Federal Republic of Germany.
Environ Res. 1992 Dec;59(2):476-84. doi: 10.1016/s0013-9351(05)80050-7.
DNA damage was detected by nucleoid sedimentation in human lymphocytes stimulated with pokeweed mitogen after exposure to enflurane. Enflurane induces DNA damage at an exposure concentration of 0.2 vol%. Higher enflurane concentrations increase the rate of DNA damage. The DNA damage seen after exposure to enflurane concentrations of 0.2 and 3.0% vol is comparable to damage after X-radiation of 0.1 and 0.7 Gy. DNA single-strand breaks can be demonstrated by nucleoid sedimentation and can indicate damage before DNA repair begins. Therefore, detected DNA single-strand breaks may be reversible. However, DNA repair is not always successful and an increased number of DNA single-strand breaks could lead to irreversible DNA damage. The method of nucleoid sedimentation helps to show DNA damage in proliferating cells after exposure to volatile anesthetics or therapeutic gases.
在用商陆有丝分裂原刺激的人淋巴细胞中，通过核仁沉降法检测暴露于恩氟烷后的DNA损伤。恩氟烷在0.2%（体积）的暴露浓度下会诱导DNA损伤。更高的恩氟烷浓度会增加DNA损伤的速率。暴露于0.2%和3.0%（体积）恩氟烷浓度后所观察到的DNA损伤与0.1和0.7戈瑞X射线照射后的损伤相当。DNA单链断裂可通过核仁沉降法证实，并且可在DNA修复开始之前指示损伤。因此，检测到的DNA单链断裂可能是可逆的。然而，DNA修复并不总是成功的，DNA单链断裂数量的增加可能导致不可逆的DNA损伤。核仁沉降法有助于显示暴露于挥发性麻醉剂或治疗性气体后增殖细胞中的DNA损伤。
