Ramussen J P, Taylor A H, Ma L-J, Purcell S, Kempken F, Catcheside D E A
School of Biological Sciences, Flinders University, P.O. Box 2100, SA 5001 Adelaide, Australia.
Fungal Genet Biol. 2004 Jan;41(1):52-61. doi: 10.1016/j.fgb.2003.09.001.
Guest is a transposable element of the Tc1/mariner superfamily with 30-40bp terminal inverted repeats and a TA dinucleotide target site duplication. Guest was originally discovered in the St. Lawrence 74A laboratory strain of the filamentous fungus Neurospora crassa. In this report, Guest iterations subcloned from a cosmid library of the Oakridge 74A strain were used to design PCR primers that permitted the detection of Guest in wild isolates of N. crassa. Guest is present in N. crassa as multiple copies ranging between 100bp and 2.4kb and is present in the mating type locus of several Neurospora species. Bioinformatic analysis of the entire N. crassa genome (Oakridge 74A strain) detected 48 Guest iterations. All iterations appeared to have been inactivated either by repeat-induced point mutation or sequence deletion, with the majority being remnants less than 400bp in length. The possible involvement of Guest in the evolution of the variable region that flanks the mating type idiomorphs in several Neurospora species is discussed.
Guest是Tc1/水手超家族的一种转座元件，具有30 - 40bp的末端反向重复序列和TA二核苷酸靶位点重复序列。Guest最初是在丝状真菌粗糙脉孢菌的圣劳伦斯74A实验室菌株中发现的。在本报告中，从奥克里奇74A菌株的黏粒文库中克隆的Guest重复序列被用于设计PCR引物，这些引物可用于检测粗糙脉孢菌野生分离株中的Guest。Guest以100bp到2.4kb之间的多个拷贝存在于粗糙脉孢菌中，并且存在于几种脉孢菌物种的交配型位点中。对粗糙脉孢菌整个基因组（奥克里奇74A菌株）的生物信息学分析检测到48个Guest重复序列。所有重复序列似乎都已通过重复诱导的点突变或序列缺失而失活，大多数是长度小于400bp的残余序列。本文还讨论了Guest在几种脉孢菌物种交配型特异序列侧翼可变区进化中的可能作用。
