从粗糙脉孢菌中分离出一种转座元件。

Kinsey J A, Helber J

Department of Microbiology, Molecular Genetics, and Immunology, University of Kansas Medical School, Kansas City 66103.

Proc Natl Acad Sci U S A. 1989 Mar;86(6):1929-33. doi: 10.1073/pnas.86.6.1929.

A Neurospora crassa strain from Adiopodoumé, Ivory Coast, contains multiple copies of a transposable element, Tad. The element was detected as a 7-kilobase insertion in two independently isolated spontaneous forward mutants of the am (glutamate dehydrogenase) gene. Laboratory strains do not contain Tad. All progeny from crosses of the Adiopodoumé strain to laboratory strains contain multiple copies. When the element was inserted in am, target sequences of 14 and 17 base pairs were duplicated in the two cases analyzed. One mutation, caused by the insertion of Tad at the beginning of the am coding sequence, is genetically stable. The other mutation, caused by insertion upstream of the transcriptional start site, has a reversion frequency of 2.5 x 10(-3). Precise excisions of Tad have not been found.

来自象牙海岸阿迪奥波杜梅的粗糙脉孢菌菌株含有多个转座元件Tad的拷贝。该元件在am（谷氨酸脱氢酶）基因的两个独立分离的自发正向突变体中被检测为一个7千碱基的插入片段。实验室菌株不含Tad。阿迪奥波杜梅菌株与实验室菌株杂交产生的所有后代都含有多个拷贝。当该元件插入am基因时，在分析的两种情况下，分别有14和17个碱基对的靶序列发生了重复。由Tad插入am编码序列起始处引起的一个突变在遗传上是稳定的。另一个由插入转录起始位点上游引起的突变，其回复频率为2.5×10⁻³。尚未发现Tad的精确切除情况。