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A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.

作者信息

Wimmer C, Doye V, Grandi P, Nehrbass U, Hurt E C

机构信息

EMBL, Heidelberg, Germany.

出版信息

EMBO J. 1992 Dec;11(13):5051-61. doi: 10.1002/j.1460-2075.1992.tb05612.x.

DOI:10.1002/j.1460-2075.1992.tb05612.x
PMID:1464327
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC556983/
Abstract

NSP1 is a nuclear pore protein (nucleoporin) essential for cell growth. To identify the components that functionally interact with NSP1 in the living cell, we developed a genetic screen for mutants that are lethal in a genetic background of mutated, but not wild type NSP1. Fourteen synthetic lethal mutants were obtained, belonging to at least four different complementation groups. The genes of two complementation groups, NSP116 and NSP49, were cloned. Like the previously described nucleoporins, these genes encode proteins with many repeat sequences. NSP116 and NSP49, however, contain a new repetitive sequence motif 'GLFG', which classifies them as a subclass of nucleoporins. NSP116 and NSP49, tagged with the IgG binding domain of protein A and expressed in yeast, are located at the nuclear envelope. These data provide in vivo evidence that distinct subclasses of nucleoporins physically interact or share overlapping function in nuclear pore complexes.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/525d91b504a1/emboj00098-0383-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/8bd9e0b22396/emboj00098-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/617b99ba9a06/emboj00098-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/53f7b1df54a4/emboj00098-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/e32de1fa740f/emboj00098-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/5e212e4fbe2c/emboj00098-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/525d91b504a1/emboj00098-0383-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/8bd9e0b22396/emboj00098-0378-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/617b99ba9a06/emboj00098-0379-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/53f7b1df54a4/emboj00098-0379-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/e32de1fa740f/emboj00098-0380-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/5e212e4fbe2c/emboj00098-0381-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f78f/556983/525d91b504a1/emboj00098-0383-a.jpg

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A new subclass of nucleoporins that functionally interact with nuclear pore protein NSP1.
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本文引用的文献

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A large particle associated with the perimeter of the nuclear pore complex.一种与核孔复合体边缘相关的大颗粒。
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A comprehensive set of sequence analysis programs for the VAX.一套适用于VAX的综合序列分析程序。
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A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.酵母中缺乏乳清苷-5'-磷酸脱羧酶活性的突变体的正向选择：5-氟乳清酸抗性。
核战争：病原体对核孔复合体及核功能的操控
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The Saccharomyces cerevisiae Spo7 basic tail is required for Nem1-Spo7/Pah1 phosphatase cascade function in lipid synthesis.酿酒酵母 Spo7 碱性尾部对 Nem1-Spo7/Pah1 磷酸酶级联在脂质合成中的功能是必需的。
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A simple thermodynamic description of phase separation of Nup98 FG domains.Nup98FG 结构域相分离的简单热力学描述。
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A conserved tryptophan within the WRDPLVDID domain of yeast Pah1 phosphatidate phosphatase is required for its function in lipid metabolism.酵母Pah1磷脂酸磷酸酶的WRDPLVDID结构域内一个保守的色氨酸对于其在脂质代谢中的功能是必需的。
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Yeast -encoded phosphatidate phosphatase controls the expression of -encoded phosphatidylserine synthase for membrane phospholipid synthesis.酵母编码的磷脂酸磷酸酶控制膜磷脂合成中编码磷脂酰丝氨酸合酶的表达。
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Phosphorylation of Dgk1 Diacylglycerol Kinase by Casein Kinase II Regulates Phosphatidic Acid Production in Saccharomyces cerevisiae.酪蛋白激酶II对二酰甘油激酶Dgk1的磷酸化作用调控酿酒酵母中磷脂酸的产生。
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Genetic analysis of the mitotic transmission of minichromosomes.微型染色体有丝分裂传递的遗传分析。
Cell. 1985 Feb;40(2):393-403. doi: 10.1016/0092-8674(85)90153-9.
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Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores.一种与核孔结合的凝集素对体外核转运的抑制作用。
J Cell Biol. 1987 Feb;104(2):189-200. doi: 10.1083/jcb.104.2.189.
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Nuclear pore complex glycoproteins contain cytoplasmically disposed O-linked N-acetylglucosamine.核孔复合体糖蛋白含有位于细胞质中的O-连接的N-乙酰葡糖胺。
J Cell Biol. 1987 May;104(5):1157-64. doi: 10.1083/jcb.104.5.1157.
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Protein import into the cell nucleus.蛋白质导入细胞核。
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