Polvani Simone, Masi Alessio, Pillozzi Serena, Gragnani Laura, Crociani Olivia, Olivotto Massimo, Becchetti Andrea, Wanke Enzo, Arcangeli Annarosa
Department of Experimental Pathology and Oncology, University of Firenze, Viale G.B.Morgagni 50, 50134, Florence, Italy.
Gene Expr Patterns. 2003 Dec;3(6):767-76. doi: 10.1016/s1567-133x(03)00124-8.
Deciphering the expression pattern of K+ channel encoding genes during development can help in the understanding of the establishment of cellular excitability and unravel the molecular mechanisms of neuromuscular diseases. We focused our attention on genes belonging to the erg family, which is deeply involved in the control of neuromuscular excitability in Drosophila flies and possibly other organisms. Both in situ hybridisation and RNase Protection Assay experiments were used to study the expression pattern of mouse (m)erg1, m-erg2 and m-erg3 genes during mouse embryo development, to allow the pattern to be compared with their expression in the adult. M-erg1 is first expressed in the heart and in the central nervous system (CNS) of embryonic day 9.5 (E9.5) embryos; the gene appears in ganglia of the peripheral nervous system (PNS) (dorsal root (DRG) and sympathetic (SCG) ganglia, mioenteric plexus), in the neural layer of retina, skeletal muscles, gonads and gut at E13.5. In the adult m-erg1 is expressed in the heart, various structures of the CNS, DRG and retina. M-erg2 is first expressed at E9.5 in the CNS, thereafter (E13.5) in the neural layer of retina, DRG, SCG, and in the atrium. In the adult the gene is present in some restricted areas of the CNS, retina and DRG. M-erg3 displayed an expression pattern partially overlapping that of m-erg1, with a transitory expression in the developing heart as well. A detailed study of the mouse adult brain showed a peculiar expression pattern of the three genes, sometimes overlapping in different encephalic areas.
解析钾离子通道编码基因在发育过程中的表达模式，有助于理解细胞兴奋性的建立，并揭示神经肌肉疾病的分子机制。我们将注意力集中在属于erg家族的基因上，该家族在果蝇以及可能在其他生物体中，都与神经肌肉兴奋性的控制密切相关。原位杂交和核糖核酸酶保护分析实验被用于研究小鼠（m）erg1、m - erg2和m - erg3基因在小鼠胚胎发育过程中的表达模式，以便将该模式与它们在成体中的表达进行比较。M - erg1首先在胚胎第9.5天（E9.5）的心脏和中枢神经系统（CNS）中表达；在E13.5时，该基因出现在外周神经系统（PNS）的神经节（背根神经节（DRG）和交感神经节（SCG）、肌间神经丛）、视网膜神经层、骨骼肌、性腺和肠道中。在成体中，m - erg1在心脏、CNS的各种结构、DRG和视网膜中表达。M - erg2首先在E9.5时在CNS中表达，之后（E13.5）在视网膜神经层、DRG、SCG和心房中表达。在成体中，该基因存在于CNS、视网膜和DRG的一些特定区域。M - erg3的表达模式与m - erg1部分重叠，在发育中的心脏中也有短暂表达。对成年小鼠大脑的详细研究显示了这三个基因独特的表达模式，有时在不同的脑区重叠。
