Wong C K, Leung K N, Fung K P, Pang P K, Choy Y M
Department of Biochemistry, Chinese University of Hong Kong, New Territories.
Int J Immunopharmacol. 1992 Nov;14(8):1315-20. doi: 10.1016/0192-0561(92)90002-3.
Separation of mitogenic fraction (PH-I) from Pseudostellaria heterophylla and characterization of its biological activities were investigated. PH-I was isolated as an alcohol-insoluble fraction from the hot water extract obtained by heating the roots of P. heterophylla in water at 80 degrees C for 4 h. It is a water-soluble substance consisting of mainly carbohydrates (56.8%) and a small amount of proteins (7.6%). The incorporation of tritiated thymidine into the DNA of murine spleen lymphocytes could be stimulated by PH-I in a dose-dependent manner. PH-I could act as a priming agent for the release of tumor necrosis factor (TNF) in mice. Moreover, PH-I exhibited potent anti-tumor activities against Ehrlich ascites tumor (EAT) cells in vivo but not in vitro.
对太子参促有丝分裂组分（PH-I）进行了分离及其生物学活性表征的研究。PH-I是通过将太子参根在80℃水中加热4小时获得的热水提取物中分离出的醇不溶部分。它是一种主要由碳水化合物（56.8%）和少量蛋白质（7.6%）组成的水溶性物质。PH-I能以剂量依赖的方式刺激氚标记胸腺嘧啶核苷掺入小鼠脾淋巴细胞的DNA。PH-I可作为小鼠肿瘤坏死因子（TNF）释放的启动剂。此外，PH-I在体内对艾氏腹水瘤（EAT）细胞具有强大的抗肿瘤活性，但在体外则没有。
